Imperial College London
Alternate

Professor Aldo R. Boccaccini

Faculty of Engineering, Department of Materials

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 6731a.boccaccini

 
 
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Location

 

210Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zehnder:2016:10.3390/ma9110887,
author = {Zehnder, T and Freund, T and Demir, M and Detsch, R and Boccaccini, AR},
doi = {10.3390/ma9110887},
journal = {Materials},
title = {Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering},
url = {http://dx.doi.org/10.3390/ma9110887},
volume = {9},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Hydrogel optimisation for biofabrication considering shape stability/mechanical properties and cell response is challenging. One approach to tackle this issue is to combine different additive manufacturing techniques, e.g., hot-melt extruded thermoplastics together with bioplotted cell loaded hydrogels in a sequential plotting process. This method enables the fabrication of 3D constructs mechanically supported by the thermoplastic structure and biologically functionalised by the hydrogel phase. In this study, polycaprolactone (PCL) and polyethylene glycol (PEG) blend (PCL-PEG) together with alginate dialdehyde gelatine hydrogel (ADA-GEL) loaded with stromal cell line (ST2) were investigated. PCL-PEG blends were evaluated concerning plotting properties to fabricate 3D scaffolds, namely miscibility, wetting behaviour and in terms of cell response. Scaffolds were characterised considering pore size, porosity, strut width, degradation behaviour and mechanical stability. Blends showed improved hydrophilicity and cell response with PEG blending increasing the degradation and decreasing the mechanical properties of the scaffolds. Hybrid constructs with PCL-PEG blend and ADA-GEL were fabricated. Cell viability, distribution, morphology and interaction of cells with the support structure were analysed. Increased degradation of the thermoplastic support structure and proliferation of the cells not only in the hydrogel, but also on the thermoplastic phase, indicates the potential of this novel material combination for biofabricating 3D tissue engineering scaffolds.
AU  - Zehnder,T
AU  - Freund,T
AU  - Demir,M
AU  - Detsch,R
AU  - Boccaccini,AR
DO  - 10.3390/ma9110887
PY  - 2016///
SN  - 1996-1944
TI  - Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering
T2  - Materials
UR  - http://dx.doi.org/10.3390/ma9110887
UR  - http://hdl.handle.net/10044/1/54060
VL  - 9
ER  -
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