Imperial College London
Alternate

Professor Alan Boobis OBE

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor of Toxicology
 
 
 
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Contact

 

a.boobis Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhu:2009:10.1289/ehp.0800109,
author = {Zhu, Z and Edwards, RJ and Boobis, AR},
doi = {10.1289/ehp.0800109},
journal = {Environmental Health Perspectives},
pages = {928--934},
title = {Increased Expression of Histone Proteins during Estrogen-Mediated Cell Proliferation},
url = {http://dx.doi.org/10.1289/ehp.0800109},
volume = {117},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: There is concern about the potential risk posed by compounds with estrogen-like activity present in the environment. As previous studies have shown that combined exposure to such compounds results in dose additivity, it should be possible to assess estrogen exposure with suitable biomarkers of effect.Objectives: Our goal was to identify candidate protein biomarkers of effect for estrogenic compounds.Methods: In the search for biomarkers, we assessed the effect of several estrogenic compounds on the expression profile of proteins in breast-derived cell lines varying in their estrogen receptor (ER) phenotype using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. We identified responsive proteins, after separating them by SDS-polyacrylamide gel electrophoresis, and analyzing the trypsin-digested proteins by tandem mass spectrometry.Results: The estrogenic compounds 17β-estradiol, genistein, bisphenol A, and endosulfan produced similar protein profile changes in MCF-7 cells (phenotype: ERα+/ERβ+), but had no effect on MDA-MB-231 (ERα−/ERβ+), MCF-10F (ERα−/ERβ+), or MCF-10A (ERα−/ERβ−) cells. The most responsive proteins in MCF-7 cells were identified as histones H2A, H2B, H3, and H4. Histone levels were not increased in cell lines that showed no proliferative response to estrogens despite their rapid intrinsic growth rate in culture.Conclusion: Our results indicate that ER-mediated cell proliferation results in up-regulation of core histone proteins.
AU  - Zhu,Z
AU  - Edwards,RJ
AU  - Boobis,AR
DO  - 10.1289/ehp.0800109
EP  - 934
PY  - 2009///
SN  - 0091-6765
SP  - 928
TI  - Increased Expression of Histone Proteins during Estrogen-Mediated Cell Proliferation
T2  - Environmental Health Perspectives
UR  - http://dx.doi.org/10.1289/ehp.0800109
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000266462600029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/48944
VL  - 117
ER  -
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