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@article{Topouzi:2017:10.1111/exd.13368,
author = {Topouzi, H and Logan, N and Williams, G and Higgins, C},
doi = {10.1111/exd.13368},
journal = {Experimental Dermatology},
pages = {491--496},
title = {Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles},
url = {http://dx.doi.org/10.1111/exd.13368},
volume = {26},
year = {2017}
}

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TY  - JOUR
AB  - The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension. As such, to grow human dermal papilla cells in vitro, the papilla has to first be isolated via a micro-dissection approach from the follicle. In this article we describe the micro-dissection and culture methods, which we use within our laboratory, for the study of human dermal papilla cells.
AU  - Topouzi,H
AU  - Logan,N
AU  - Williams,G
AU  - Higgins,C
DO  - 10.1111/exd.13368
EP  - 496
PY  - 2017///
SN  - 1600-0625
SP  - 491
TI  - Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles
T2  - Experimental Dermatology
UR  - http://dx.doi.org/10.1111/exd.13368
UR  - http://hdl.handle.net/10044/1/48214
VL  - 26
ER  -

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