Imperial College London
Alternate

Professor Francis Drobniewski

Faculty of Medicine, Department of Infectious Disease

Chair in Global Health and Tuberculosis
 
 
 
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Contact

 

f.drobniewski

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Broda:2018:10.1007/s10096-018-3246-2,
author = {Broda, A and Nikolayevskyy, V and Casali, N and Khan, H and Bowker, R and Blackwell, G and Patel, B and Hume, J and Hussain, W and Drobniewski, F},
doi = {10.1007/s10096-018-3246-2},
journal = {European Journal of Clinical Microbiology and Infectious Diseases},
pages = {1273--1279},
title = {Experimental platform utilising melting curve technology for detection of mutations in Mycobacterium tuberculosis isolates},
url = {http://dx.doi.org/10.1007/s10096-018-3246-2},
volume = {37},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tuberculosis (TB) remains one of the most deadly infections with approximately a quarter of cases not being identified and/or treated mainly due to a lack of resources. Rapid detection of TB or drug-resistant TB enables timely adequate treatment and is a cornerstone of effective TB management. We evaluated the analytical performance of a single-tube assay for multidrug-resistant TB (MDR-TB) on an experimental platform utilising RT-PCR and melting curve analysis that could potentially be operated as a point-of-care (PoC) test in resource-constrained settings with a high burden of TB. Firstly, we developed and evaluated the prototype MDR-TB assay using specimens extracted from well-characterised TB isolates with a variety of distinct rifampicin and isoniazid resistance conferring mutations and nontuberculous Mycobacteria (NTM) strains. Secondly, we validated the experimental platform using 98 clinical sputum samples from pulmonary TB patients collected in high MDR-TB settings. The sensitivity of the platform for TB detection in clinical specimens was 75% for smear-negative and 92.6% for smear-positive sputum samples. The sensitivity of detection for rifampicin and isoniazid resistance was 88.9 and 96.0% and specificity was 87.5 and 100%, respectively. Observed limitations in sensitivity and specificity could be resolved by adjusting the sample preparation methodology and melting curve recognition algorithm. Overall technology could be considered a promising PoC methodology especially in resource-constrained settings based on its combined accuracy, convenience, simplicity, speed, and cost characteristics.
AU  - Broda,A
AU  - Nikolayevskyy,V
AU  - Casali,N
AU  - Khan,H
AU  - Bowker,R
AU  - Blackwell,G
AU  - Patel,B
AU  - Hume,J
AU  - Hussain,W
AU  - Drobniewski,F
DO  - 10.1007/s10096-018-3246-2
EP  - 1279
PY  - 2018///
SN  - 0934-9723
SP  - 1273
TI  - Experimental platform utilising melting curve technology for detection of mutations in Mycobacterium tuberculosis isolates
T2  - European Journal of Clinical Microbiology and Infectious Diseases
UR  - http://dx.doi.org/10.1007/s10096-018-3246-2
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29675789
UR  - http://hdl.handle.net/10044/1/59217
VL  - 37
ER  -
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