Imperial College London
Alternate

ProfessorGeoffBaldwin

Faculty of Natural Sciences, Department of Life Sciences

Professor of Synthetic and Molecular Biology
 
 
 
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Contact

 

+44 (0)20 7594 5288g.baldwin

 
 
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Location

 

508Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haines:2019:synbio/ysz019,
author = {Haines, M and Storch, M and Oyarzun, D and Stan, G and Baldwin, G},
doi = {synbio/ysz019},
journal = {Synthetic Biology},
pages = {1--10},
title = {Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR)},
url = {http://dx.doi.org/10.1093/synbio/ysz019},
volume = {4},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental outcomes and unknown functionality of enriched sequences in vivo. To address the first of these limitations we developed Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR). LigASERR is scalable, amenable to automation and requires less time to implement compared to alternative methods. To improve the predictability of experiments, we modelled the underlying selection process, predicting experimental outcomes based on sequence and population parameters. We applied this new methodology and model to the enrichment of a known, in vitro-selected sequence from a bespoke library. Prior to implementing selection, conditions were optimised and target sequence dynamics accurately predicted for the majority of the experiment. In addition to enriching the target sequence, we identified two new, theophylline-activated ribozymes. Notably, all three sequences yielded riboswitches functional in Escherichia coli, suggesting LigASERR and similar in vitro selection methods can be utilised for generating functional riboswitches in this organism.
AU  - Haines,M
AU  - Storch,M
AU  - Oyarzun,D
AU  - Stan,G
AU  - Baldwin,G
DO  - synbio/ysz019
EP  - 10
PY  - 2019///
SN  - 2397-7000
SP  - 1
TI  - Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR)
T2  - Synthetic Biology
UR  - http://dx.doi.org/10.1093/synbio/ysz019
UR  - https://academic.oup.com/synbio/advance-article/doi/10.1093/synbio/ysz019/5529681
UR  - http://hdl.handle.net/10044/1/71866
VL  - 4
ER  -
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