Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wan:2024:infdis/jiae019,
author = {Wan, Y and Myall, AC and Boonyasiri, A and Bolt, F and Ledda, A and Mookerjee, S and Weiße, AY and Getino, M and Turton, JF and Abbas, H and Prakapaite, R and Sabnis, A and Abdolrasouli, A and Malpartida-Cardenas, K and Miglietta, L and Donaldson, H and Gilchrist, M and Hopkins, KL and Ellington, MJ and Otter, JA and Larrouy-Maumus, G and Edwards, AM and Rodriguez-Manzano, J and Didelot, X and Barahona, M and Holmes, AH and Jauneikaite, E and Davies, F},
doi = {infdis/jiae019},
journal = {J Infect Dis},
title = {Integrated analysis of patient networks and plasmid genomes reveals a regional, multi-species outbreak of carbapenemase-producing Enterobacterales carrying both blaIMP and mcr-9 genes.},
url = {http://dx.doi.org/10.1093/infdis/jiae019},
year = {2024}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Carbapenemase-producing Enterobacterales (CPE) are challenging in healthcare, with resistance to multiple classes of antibiotics. This study describes the emergence of IMP-encoding CPE amongst diverse Enterobacterales species between 2016 and 2019 across a London regional network. METHODS: We performed a network analysis of patient pathways, using electronic health records, to identify contacts between IMP-encoding CPE positive patients. Genomes of IMP-encoding CPE isolates were overlayed with patient contacts to imply potential transmission events. RESULTS: Genomic analysis of 84 Enterobacterales isolates revealed diverse species (predominantly Klebsiella spp, Enterobacter spp, E. coli); 86% (72/84) harboured an IncHI2 plasmid carrying blaIMP and colistin resistance gene mcr-9 (68/72). Phylogenetic analysis of IncHI2 plasmids identified three lineages showing significant association with patient contacts and movements between four hospital sites and across medical specialities, which was missed on initial investigations. CONCLUSIONS: Combined, our patient network and plasmid analyses demonstrate an interspecies, plasmid-mediated outbreak of blaIMPCPE, which remained unidentified during standard investigations. With DNA sequencing and multi-modal data incorporation, the outbreak investigation approach proposed here provides a framework for real-time identification of key factors causing pathogen spread. Plasmid-level outbreak analysis reveals that resistance spread may be wider than suspected, allowing more interventions to stop transmission within hospital networks.
AU  - Wan,Y
AU  - Myall,AC
AU  - Boonyasiri,A
AU  - Bolt,F
AU  - Ledda,A
AU  - Mookerjee,S
AU  - Weiße,AY
AU  - Getino,M
AU  - Turton,JF
AU  - Abbas,H
AU  - Prakapaite,R
AU  - Sabnis,A
AU  - Abdolrasouli,A
AU  - Malpartida-Cardenas,K
AU  - Miglietta,L
AU  - Donaldson,H
AU  - Gilchrist,M
AU  - Hopkins,KL
AU  - Ellington,MJ
AU  - Otter,JA
AU  - Larrouy-Maumus,G
AU  - Edwards,AM
AU  - Rodriguez-Manzano,J
AU  - Didelot,X
AU  - Barahona,M
AU  - Holmes,AH
AU  - Jauneikaite,E
AU  - Davies,F
DO  - infdis/jiae019
PY  - 2024///
TI  - Integrated analysis of patient networks and plasmid genomes reveals a regional, multi-species outbreak of carbapenemase-producing Enterobacterales carrying both blaIMP and mcr-9 genes.
T2  - J Infect Dis
UR  - http://dx.doi.org/10.1093/infdis/jiae019
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38245822
ER  -
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