Publications
246 results found
Calcagno-Pizarelli AM, Negrete-Urtasun S, Denison SH, et al., 2007, Establishment of the ambient pH signaling complex in <i>Aspergillus nidulans</i>:: PalI assists plasma membrane localization of PalH, EUKARYOTIC CELL, Vol: 6, Pages: 2365-2375, ISSN: 1535-9778
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- Citations: 72
Hervas-Aguilar A, Rodriguez JM, Tilburn J, et al., 2007, Evidence for the direct involvement of the proteasome in the proteolytic processing of the <i>Aspergillus</i> <i>nidulans</i> zinc finger transcription factor PacC, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 282, Pages: 34735-34747, ISSN: 0021-9258
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- Citations: 65
Galindo A, Hervas-Aguilar A, Rodriguez-Galan O, et al., 2007, PalC, one of two Bro1 domain proteins in the fungal pH signaling pathway, localizes to cortical structures and binds Vps32, Traffic, Vol: 8, Pages: 1346-1364
Penas MM, Hervas-Aguilar A, Munera-Huertas T, et al., 2007, Further characterization of the signaling proteolysis step in the <i>Aspergillus nidulans</i> pH signal transduction pathway, EUKARYOTIC CELL, Vol: 6, Pages: 960-970, ISSN: 1535-9778
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- Citations: 53
Schrettl M, Bignell E, Kragl C, et al., 2007, Distinct Roles for Intra- and Extracellular Siderophores during Aspergillus fumigatus Infection, PLoS Pathogens, Vol: 3
Siderophore biosynthesis by the highly lethal mould Aspergillus fumigatus is essential for virulence, but non-existent in humans, presenting a rare opportunity to strategize therapeutically against this pathogen. We have previously demonstrated that A. fumigatus excretes fusarinine C and triacetylfusarinine C to capture extracellular iron, and uses ferricrocin for hyphal iron storage. Here, we delineate pathways of intra- and extracellular siderophore biosynthesis and show that A. fumigatus synthesizes a developmentally regulated fourth siderophore, termed hydroxyferricrocin, employed for conidial iron storage. By inactivation of the nonribosomal peptide synthetase SidC, we demonstrate that the intracellular siderophores are required for germ tube formation, asexual sporulation, resistance to oxidative stress, catalase A activity, and virulence. Restoration of the conidial hydroxyferricrocin content partially rescues the virulence of the apathogenic siderophore null mutant DeltasidA, demonstrating an important role for the conidial siderophore during initiation of infection. Abrogation of extracellular siderophore biosynthesis following inactivation of the acyl transferase SidF or the nonribosomal peptide synthetase SidD leads to complete dependence upon reductive iron assimilation for growth under iron-limiting conditions, partial sensitivity to oxidative stress, and significantly reduced virulence, despite normal germ tube formation. Our findings reveal distinct cellular and disease-related roles for intra- and extracellular siderophores during mammalian Aspergillus infection.
Morozov IY, Negrete-Urtasun S, Tilburn J, et al., 2006, Nonsense-mediated mRNA decay mutation in <i>Aspergillus nidulans</i>, EUKARYOTIC CELL, Vol: 5, Pages: 1838-1846, ISSN: 1535-9778
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- Citations: 16
Meyer V, Spielvogel A, Funk L, et al., 2005, Alkaline pH-induced up-regulation of the <i>afp</i> gene encoding the antifungal protein (AFP) of <i>Aspergillus giganteus</i> is not mediated by the transcription factor PacC:: possible involvement of calcineurin, MOLECULAR GENETICS AND GENOMICS, Vol: 274, Pages: 295-306, ISSN: 1617-4615
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- Citations: 9
Herranz S, Rodríguez JM, Bussink HJ, et al., 2005, Arrestin-related proteins mediate pH signaling in fungi, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 102, Pages: 12141-12146, ISSN: 0027-8424
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- Citations: 117
Bignell E, Negrete-Urtasun S, Calcagno AM, et al., 2005, Virulence comparisons of <i>Aspergillus nidulans</i> mutants are confounded by the inflammatory response of p47<SUP><i>phox</i>-/-</SUP> mice, INFECTION AND IMMUNITY, Vol: 73, Pages: 5204-5207, ISSN: 0019-9567
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- Citations: 24
Bignell E, Negrete-Urtasun S, Calcagno AM, et al., 2005, The <i>Aspergillus</i> pH-responsive transcription factor PacC regulates virulence, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 1072-1084, ISSN: 0950-382X
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- Citations: 86
Tilburn J, Sanchez-Ferrero JC, Reoyo E, et al., 2005, Mutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 family members, Genetics, Vol: 171, Pages: 393-401
Espeso EA, Cobeno L, Arst HN Jr, 2005, Discrepancies between recombination frequencies and physical distances in Aspergillus nidulans: implications for gene identification, Genetics, Vol: 171, Pages: 835-838
Sarkar S, Caddick MX, Bignell E, et al., 2004, Regulation of gene expression by ambient pH in Aspergillus: genes expressed at acidic pH (vol 24, pg 360, 1996), BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 32, Pages: 1133-1133, ISSN: 0300-5127
Schrettl M, Bignell E, Kragl C, et al., 2004, Siderophore biosynthesis but not reductive iron assimilation is essential for <i>Aspergillus fumigates</i> virulence, JOURNAL OF EXPERIMENTAL MEDICINE, Vol: 200, Pages: 1213-1219, ISSN: 0022-1007
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- Citations: 393
Tanzer MM, Arst HN, Skalchunes AR, et al., 2004, Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi (vol3, pg 160, 2003), FUNCTIONAL & INTEGRATIVE GENOMICS, Vol: 4, Pages: 67-69, ISSN: 1438-793X
Sarkar S, Caddick MX, Bignell E, et al., 2004, Erratum: Regulation of gene expression by ambient pH in Aspergillus: Genes expressed at acidic pH (Biochemical Society Transactions (1996) 24, Part 2 (360-363)), Biochemical Society Transactions, Vol: 32, ISSN: 0300-5127
Arst HN Jr, Tilburn J, 2004, Regulation of gene expression by ambient pH., The Mycota Vol. III. Biochemistry and Molecular Biology., Editors: Brambl, Marzluf, Brambl, Marzluf, Brambl, Marzluf, Berlin, Publisher: Springer-Verlag, Pages: 121-128, ISBN: 9783540426301
Peñalva MA, Arst HN, 2004, Recent advances in the characterization of ambient pH regulation of gene expression in filamentour fungi and yeasts, ANNUAL REVIEW OF MICROBIOLOGY, Vol: 58, Pages: 425-451, ISSN: 0066-4227
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- Citations: 158
Fernández-Martínez J, Brown CV, Díez E, et al., 2003, Overlap of nuclear localisation signal and specific DNA-binding residues within the zinc finger domain of PacC, JOURNAL OF MOLECULAR BIOLOGY, Vol: 334, Pages: 667-684, ISSN: 0022-2836
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- Citations: 65
Tanzer MM, Arst HN, Skalchunes AR, et al., 2003, Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi., Funct Integr Genomics, Vol: 3, Pages: 160-170, ISSN: 1438-793X
We describe a method for gene function discovery and chemical mode-of-action analysis via nutrient utilization using a high throughput Nutritional Profiling platform suitable for filamentous microorganisms. We have optimized the growth conditions for each fungal species to produce reproducible optical density growth measurements in microtiter plates. We validated the Nutritional Profiling platform using a nitrogen source utilization assay to analyze 21 Aspergillus nidulans strains with mutations in the master nitrogen regulatory gene, areA. Analysis of these data accurately reproduced expected results and provided new data to demonstrate that this platform is suitable for fine level phenotyping of filamentous fungi. Next, we analyzed the differential responses of two fungal species to a glutamine synthetase inhibitor, illustrating chemical mode-of-action analysis. Finally, a comparative phenotypic study was performed to characterize carbon catabolite repression in four fungal species using a carbon source utilization assay. The results demonstrate differentiation between two Aspergillus species and two diverse plant pathogens and provide a wealth of new data on fungal nutrient utilization. Thus, these assays can be used for gene function and chemical mode-of-action analysis at the whole organism level as well as interspecies comparisons in a variety of filamentous fungi. Additionally, because uniform distribution of growth within wells is maintained, comparisons between yeast and filamentous forms of a single organism can be performed.
Arst HN, Peñalva MA, 2003, Recognizing gene regulation by ambient pH, FUNGAL GENETICS AND BIOLOGY, Vol: 40, Pages: 1-3, ISSN: 1087-1845
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- Citations: 12
Arst HN, Nicholas HB, Caddick MX, 2003, Re:: Watts et al. proteins 2002;48:161-168, PROTEINS-STRUCTURE FUNCTION AND GENETICS, Vol: 52, Pages: 125-128, ISSN: 0887-3585
Flipphi M, van de Vondervoort PJI, Ruijter GJG, et al., 2003, Onset of carbon catabolite repression in <i>Aspergillus nidulans</i> -: Parallel involvement of hexokinase and glucokinase in sugar signaling, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 278, Pages: 11849-11857, ISSN: 0021-9258
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- Citations: 72
Arst HN, Peñalva MA, 2003, pH regulation in <i>Aspergillus</i> and parallels with higher eukaryotic regulatory systems, TRENDS IN GENETICS, Vol: 19, Pages: 224-231, ISSN: 0168-9525
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- Citations: 102
Vincent O, Rainbow L, Tilburn J, et al., 2003, YPXL/I is a protein interaction motif recognized by <i>Aspergillus</i> PalA and its human homologue, AIP1/Alix, MOLECULAR AND CELLULAR BIOLOGY, Vol: 23, Pages: 1647-1655, ISSN: 0270-7306
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- Citations: 135
Peñalva MA, Arst HN, 2002, Regulation of gene expression by ambient pH in filamentous fungi and yeasts, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Vol: 66, Pages: 426-+, ISSN: 1092-2172
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- Citations: 239
Peñalva MA, Arst HN, 2002, Regulation of gene expression by ambient pH in filamentous fungi and yeasts., Microbiol Mol Biol Rev, Vol: 66, Pages: 426-446, ISSN: 1092-2172
Life, as we know it, is water based. Exposure to hydroxonium and hydroxide ions is constant and ubiquitous, and the evolutionary pressure to respond appropriately to these ions is likely to be intense. Fungi respond to their environments by tailoring their output of activities destined for the cell surface or beyond to the ambient pH. We are beginning to glimpse how they sense ambient pH and transmit this information to the transcription factor, whose roles ensure that a suitable collection of gene products will be made. Although relatively little is known about pH signal transduction itself, its consequences for the cognate transcription factor are much clearer. Intriguingly, homologues of components of this system mediating the regulation of fungal gene expression by ambient pH are to be found in the animal kingdom. The potential applied importance of this regulatory system lies in its key role in fungal pathogenicity of animals and plants and in its control of fungal production of toxins, antibiotics, and secreted enzymes.
Díez E, Alvaro J, Espeso EA, et al., 2002, Activation of the <i>Aspergillus</i> PacC zinc finger transcription factor requires two proteolytic steps, EMBO JOURNAL, Vol: 21, Pages: 1350-1359, ISSN: 0261-4189
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- Citations: 104
, 2001, Riboflavin, overproduced during sporulation of <i>Ashbya gossypii</i>, protects its hyaline spores against ultraviolet light, ENVIRONMENTAL MICROBIOLOGY, Vol: 3, Pages: 545-550, ISSN: 1462-2912
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- Citations: 47
Conlon H, Zadra I, Haas H, et al., 2001, The <i>Aspergillus nidulans</i> GATA transcription factor gene <i>areB</i> encodes at least three proteins and features three classes of mutation, MOLECULAR MICROBIOLOGY, Vol: 40, Pages: 361-375, ISSN: 0950-382X
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- Citations: 39
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