Imperial College London

Professor Molly Stevens FREng

Faculty of EngineeringDepartment of Materials

Prof of Biomedical Materials&Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chiappini:2015:10.1038/nmat4249,
author = {Chiappini, C and De, Rosa E and Martinez, JO and Liu, X and Steele, J and Stevens, MM and Tasciotti, E},
doi = {10.1038/nmat4249},
journal = {Nature Materials},
pages = {532--539},
title = {Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.},
url = {http://dx.doi.org/10.1038/nmat4249},
volume = {14},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The controlled delivery of nucleic acids to selected tissues remains an inefficient process mired by low transfection efficacy, poor scalability because of varying efficiency with cell type and location, and questionable safety as a result of toxicity issues arising from the typical materials and procedures employed. High efficiency and minimal toxicity in vitro has been shown for intracellular delivery of nuclei acids by using nanoneedles, yet extending these characteristics to in vivo delivery has been difficult, as current interfacing strategies rely on complex equipment or active cell internalization through prolonged interfacing. Here, we show that a tunable array of biodegradable nanoneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.
AU - Chiappini,C
AU - De,Rosa E
AU - Martinez,JO
AU - Liu,X
AU - Steele,J
AU - Stevens,MM
AU - Tasciotti,E
DO - 10.1038/nmat4249
EP - 539
PY - 2015///
SN - 1476-4660
SP - 532
TI - Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.
T2 - Nature Materials
UR - http://dx.doi.org/10.1038/nmat4249
UR - http://hdl.handle.net/10044/1/21906
VL - 14
ER -