Imperial College London
Alternate

ProfessorMarinvan Heel

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 5316m.vanheel Website

 
 
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Assistant

 

Dr Audrey Geffen +44 (0)20 7594 5323

 
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Location

 

G20Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stark:1997:10.1016/s0092-8674(00)81854-1,
author = {Stark, H and Orlova, EV and Rinke-Appel, J and Jünke, N and Mueller, F and Rodnina, M and Wintermeyer, W and Brimacombe, R and van, Heel M},
doi = {10.1016/s0092-8674(00)81854-1},
journal = {Cell},
pages = {19--28},
title = {Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy.},
url = {http://dx.doi.org/10.1016/s0092-8674(00)81854-1},
volume = {88},
year = {1997}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The three-dimensional structure of the translating 70S E. coli ribosome is presented in its two main conformations: the pretranslocational and the posttranslocational states. Using electron cryomicroscopy and angular reconstitution, structures at 20 A resolution were obtained, which, when compared with our earlier reconstruction of "empty" ribosomes, showed densities corresponding to tRNA molecules--at the P and E sites for posttranslocational ribosomes and at the A and P sites for pretranslocational ribosomes. The P-site tRNA lies directly above the bridge connecting the two ribosomal subunits, with the A-site tRNA fitted snugly against it at an angle of approximately 50 degrees, toward the L7/L12 side of the ribosome. The E-site tRNA appears to lie between the side lobe of the 30S subunit and the L1 protuberance.
AU  - Stark,H
AU  - Orlova,EV
AU  - Rinke-Appel,J
AU  - Jünke,N
AU  - Mueller,F
AU  - Rodnina,M
AU  - Wintermeyer,W
AU  - Brimacombe,R
AU  - van,Heel M
DO  - 10.1016/s0092-8674(00)81854-1
EP  - 28
PY  - 1997///
SN  - 0092-8674
SP  - 19
TI  - Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy.
T2  - Cell
UR  - http://dx.doi.org/10.1016/s0092-8674(00)81854-1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/9019401
VL  - 88
ER  -
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