Imperial College London

Dr Nick Brooks

Faculty of Natural SciencesDepartment of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2677n.brooks Website

 
 
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Location

 

207JMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Menny:2018:10.1038/s41467-018-07653-5,
author = {Menny, A and Serna, M and Boyd, C and Gardner, S and Joseph, AP and Morgan, BP and Topf, M and Brooks, NJ and Bubeck, D},
doi = {10.1038/s41467-018-07653-5},
journal = {Nature Communications},
title = {CryoEM reveals how the complement membrane attack complex ruptures lipid bilayers},
url = {http://dx.doi.org/10.1038/s41467-018-07653-5},
volume = {9},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The membrane attack complex (MAC) is one of the immune system’s first responders. Complement proteins assemble on target membranes to form pores that lyse pathogens and impact tissue homeostasis of self-cells. How MAC disrupts the membrane barrier remains unclear. Here we use electron cryo-microscopy and flicker spectroscopy to show that MAC interacts with lipid bilayers in two distinct ways. Whereas C6 and C7 associate with the outer leaflet and reduce the energy for membrane bending, C8 and C9 traverse the bilayer increasing membrane rigidity. CryoEM reconstructions reveal plasticity of the MAC pore and demonstrate how C5b6 acts as a platform, directing assembly of a giant β-barrel whose structure is supported by a glycan scaffold. Our work provides a structural basis for understanding how β-pore forming proteins breach the membrane and reveals a mechanism for how MAC kills pathogens and regulates cell functions.
AU - Menny,A
AU - Serna,M
AU - Boyd,C
AU - Gardner,S
AU - Joseph,AP
AU - Morgan,BP
AU - Topf,M
AU - Brooks,NJ
AU - Bubeck,D
DO - 10.1038/s41467-018-07653-5
PY - 2018///
SN - 2041-1723
TI - CryoEM reveals how the complement membrane attack complex ruptures lipid bilayers
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-018-07653-5
UR - https://www.nature.com/articles/s41467-018-07653-5
UR - http://hdl.handle.net/10044/1/66336
VL - 9
ER -