Imperial College London
Alternate

ProfessorNadiaRosenthal

Faculty of Medicine, National Heart & Lung Institute

Chair in Cardiovascular Science&ScientificDirector
 
 
 
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Contact

 

+44 (0)20 7594 2737n.rosenthal

 
 
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Location

 

424W2ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pinto:2014:10.18632/aging.100669,
author = {Pinto, AR and Godwin, JW and Chandran, A and Hersey, L and Ilinykh, A and Debuque, R and Wang, L and Rosenthal, NA},
doi = {10.18632/aging.100669},
journal = {Aging-US},
pages = {399--413},
title = {Age-related changes in tissue macrophages precede cardiac functional impairment},
url = {http://dx.doi.org/10.18632/aging.100669},
volume = {6},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cardiac tissue macrophages (cTMs) are abundant in the murine heart but the extent to which the cTM phenotype changes with age is unknown. This study characterizes aging-dependent phenotypic changes in cTM subsets. Using the Cx3cr1GFP/+ mouse reporter line where GFP marks cTMs, and the tissue macrophage marker Mrc1, we show that two major cardiac tissue macrophage subsets, Mrc1−GFPhi and Mrc1+GFPhi cTMs, are present in the young (<10 week old) mouse heart, and a third subset, Mrc1+GFPlo, comprises ~50% of total Mrc1+ cTMs from 30 weeks of age. Immunostaining and functional assays show that Mrc1+ cTMs are the principal myeloid sentinels in the mouse heart and that they retain proliferative capacity throughout life. Gene expression profiles of the two Mrc1+ subsets also reveal that Mrc1+GFPlo cTMs have a decreased number of immune response genes (Cx3cr1, Lpar6, CD9, Cxcr4, Itga6 and Tgfβr1), and an increased number of fibrogenic genes (Ltc4s, Retnla, Fgfr1, Mmp9 and Ccl24), consistent with a potential role for cTMs in cardiac fibrosis. These findings identify early age-dependent gene expression changes in cTMs, with significant implications for cardiac tissue injury responses and aging-associated cardiac fibrosis.
AU  - Pinto,AR
AU  - Godwin,JW
AU  - Chandran,A
AU  - Hersey,L
AU  - Ilinykh,A
AU  - Debuque,R
AU  - Wang,L
AU  - Rosenthal,NA
DO  - 10.18632/aging.100669
EP  - 413
PY  - 2014///
SN  - 1945-4589
SP  - 399
TI  - Age-related changes in tissue macrophages precede cardiac functional impairment
T2  - Aging-US
UR  - http://dx.doi.org/10.18632/aging.100669
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336532200007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.aging-us.com/article/100669
UR  - http://hdl.handle.net/10044/1/82111
VL  - 6
ER  -
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