Imperial College London
Alternate

Professor Richard Reynolds, BSc AKC PhD

Faculty of Medicine, Department of Brain Sciences

Professor of Cellular Neurobiology
 
 
 
//

Contact

 

+44 (0)20 7594 6668r.reynolds

 
 
//

Location

 

E414Burlington DanesHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Zhang:2015:10.1523/JNEUROSCI.3552-14.2015,
author = {Zhang, A and Desmazieres, A and Zonta, B and Melrose, S and Campbell, G and Mahad, D and Li, Q and Sherman, DL and Reynolds, R and Brophy, PJ},
doi = {10.1523/JNEUROSCI.3552-14.2015},
journal = {Journal of Neuroscience},
pages = {2246--2254},
title = {Neurofascin 140 is an embryonic neuronal neurofascin isoform that promotes the assembly of the node of ranvier},
url = {http://dx.doi.org/10.1523/JNEUROSCI.3552-14.2015},
volume = {35},
year = {2015}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rapid nerve conduction in myelinated nerves requires the clustering of voltage-gated sodium channels at nodes of Ranvier. The Neurofascin (Nfasc) gene has a unique role in node formation because it encodes glial and neuronal isoforms of neurofascin (Nfasc155 and Nfasc186, respectively) with key functions in assembling the nodal macromolecular complex. A third neurofascin, Nfasc140, has also been described; however, neither the cellular origin nor function of this isoform was known. Here we show that Nfasc140 is a neuronal protein strongly expressed during mouse embryonic development. Expression of Nfasc140 persists but declines during the initial stages of node formation, in contrast to Nfasc155 and Nfasc186, which increase. Nevertheless, Nfasc140, like Nfasc186, can cluster voltage-gated sodium channels (Nav) at the developing node of Ranvier and can restore electrophysiological function independently of Nfasc155 and Nfasc186. This suggests that Nfasc140 complements the function of Nfasc155 and Nfasc186 in initial stages of the assembly and stabilization of the nodal complex. Further, Nfasc140 is reexpressed in demyelinated white matter lesions of postmortem brain tissue from human subjects with multiple sclerosis. This expands the critical role of the Nfasc gene in the function of myelinated axons and reveals further redundancy in the mechanisms required for the formation of this crucial structure in the vertebrate nervous system.
AU  - Zhang,A
AU  - Desmazieres,A
AU  - Zonta,B
AU  - Melrose,S
AU  - Campbell,G
AU  - Mahad,D
AU  - Li,Q
AU  - Sherman,DL
AU  - Reynolds,R
AU  - Brophy,PJ
DO  - 10.1523/JNEUROSCI.3552-14.2015
EP  - 2254
PY  - 2015///
SN  - 1529-2401
SP  - 2246
TI  - Neurofascin 140 is an embryonic neuronal neurofascin isoform that promotes the assembly of the node of ranvier
T2  - Journal of Neuroscience
UR  - http://dx.doi.org/10.1523/JNEUROSCI.3552-14.2015
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349671100037&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/30933
VL  - 35
ER  -
Download