Imperial College London
Alternate

ProfessorSimakAli

Faculty of Medicine, Department of Surgery & Cancer

Professor of Molecular Endocrine Oncology
 
 
 
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Contact

 

+44 (0)20 7594 2811simak.ali

 
 
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Location

 

133ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Pálinkás:2020:10.1101/2020.03.04.976977,
author = {Pálinkás, HL and Békési, A and Róna, G and Pongor, L and Tihanyi, G and Holub, E and Póti, Á and Gemma, C and Ali, S and Morten, MJ and Rothenberg, E and Pagano, M and Szüts, D and Gyrffy, B and Vértessy, BG},
doi = {10.1101/2020.03.04.976977},
publisher = {eLife Sciences Publications Ltd},
title = {Genome-wide alterations of uracil distribution patterns in human DNA upon chemotherapeutic treatments},
url = {http://dx.doi.org/10.1101/2020.03.04.976977},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>ABSTRACT</jats:title><jats:p>Numerous anti-cancer drugs perturb thymidylate biosynthesis and lead to genomic uracil incorporation contributing to their antiproliferative effect. Still, it is not yet characterized if uracil incorporations have any positional preference. Here, we aimed to uncover genome-wide alterations in uracil pattern upon drug-treatment in human cancer cell-line HCT116. We developed a straightforward U-DNA sequencing method (U-DNA-Seq) that was combined with <jats:italic>in situ</jats:italic> super-resolution imaging. Using a novel robust analysis pipeline, we found broad regions with elevated probability of uracil occurrence both in treated and non-treated cells. Correlation with chromatin markers and other genomic features shows that non-treated cells possess uracil in the late replicating constitutive heterochromatic regions, while drug treatment induced a shift of incorporated uracil towards more active/functional segments. Data were corroborated by colocalization studies via dSTORM microscopy. This approach can also be applied to study the dynamic <jats:italic>spatio-temporal</jats:italic> nature of genomic uracil.</jats:p>
AU  - Pálinkás,HL
AU  - Békési,A
AU  - Róna,G
AU  - Pongor,L
AU  - Tihanyi,G
AU  - Holub,E
AU  - Póti,Á
AU  - Gemma,C
AU  - Ali,S
AU  - Morten,MJ
AU  - Rothenberg,E
AU  - Pagano,M
AU  - Szüts,D
AU  - Gyrffy,B
AU  - Vértessy,BG
DO  - 10.1101/2020.03.04.976977
PB  - eLife Sciences Publications Ltd
PY  - 2020///
TI  - Genome-wide alterations of uracil distribution patterns in human DNA upon chemotherapeutic treatments
UR  - http://dx.doi.org/10.1101/2020.03.04.976977
ER  -
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