Research Interests and Techniques
I am interested in how neuronal properties are specified and maintained in the developing and adult nervous system. Gaining a comprehensive understanding of gene regulatory networks that specify neuronal fate will provide methods for reprogramming neuronal properties in vivo.
This research utilises the cell-type specific profiling technique TaDa (Southall et al. 2013) to map the transcriptional state, and the genomic binding of transcriptional regulators, in specific neuronal populations.
For more information, please see the lab website.
Aughey GN, Southall TD, 2016, Dam it's good! DamID profiling of protein-DNA interactions, Wiley Interdisciplinary Reviews-developmental Biology, Vol:5, ISSN:1759-7684, Pages:25-37
et al., 2016, Functional Conservation of the Glide/Gcm Regulatory Network Controlling Glia, Hemocyte, and Tendon Cell Differentiation in Drosophila, Genetics, Vol:202, ISSN:0016-6731, Pages:191-+
et al., 2016, Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing, Nature Protocols, Vol:11, ISSN:1754-2189, Pages:1586-1598
McClure CD, Southall TD, 2015, Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner, Advances in Genetics, Vol 91, Vol:91, ISSN:0065-2660, Pages:103-151
et al., 2014, Escargot maintains stemness and suppresses differentiation in Drosophila intestinal stem cells, Embo Journal, Vol:33, ISSN:0261-4189, Pages:2967-2982