Research Interests and Techniques
I am interested in how neuronal properties are specified and maintained in the developing and adult nervous system. Gaining a comprehensive understanding of gene regulatory networks that specify neuronal fate will provide methods for reprogramming neuronal properties in vivo.
This research utilises the cell-type specific profiling technique TaDa (Southall et al. 2013) to map the transcriptional state, and the genomic binding of transcriptional regulators, in specific neuronal populations.
For more information, please see the lab website.
et al., 2018, Targeted DamID reveals differential binding of mammalian pluripotency factors., Development
et al., 2018, CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo, Elife, Vol:7, ISSN:2050-084X
et al., 2017, Comprehensive Characterization of the Complex lola Locus Reveals a Novel Role in the Octopaminergic Pathway via Tyramine Beta-Hydroxylase Regulation, Cell Reports, Vol:21, ISSN:2211-1247, Pages:2911-2925
et al., 2016, Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing, Nature Protocols, Vol:11, ISSN:1754-2189, Pages:1586-1598
Aughey GN, Southall TD, 2016, Dam it's good! DamID profiling of protein-DNA interactions, Wiley Interdisciplinary Reviews-developmental Biology, Vol:5, ISSN:1759-7684, Pages:25-37