Imperial College London
Alternate

Professor Terry Tetley

Faculty of Medicine, National Heart & Lung Institute

Professor of Lung Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 2984t.tetley Website

 
 
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Location

 

109Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Katsumiti:2020:10.1038/s41598-020-77191-y,
author = {Katsumiti, A and Ruenraroengsak, P and Cajaraville, MP and Thorley, AJ and Tetley, T},
doi = {10.1038/s41598-020-77191-y},
journal = {Scientific Reports},
title = {Immortalisation of primary human alveolar epithelial cells using a non-viral vector to study respiratory bioreactivity in vitro},
url = {http://dx.doi.org/10.1038/s41598-020-77191-y},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - To overcome the scarcity of primary human alveolar epithelial cells for lung research, and the limitations of current cell lines to recapitulate the phenotype, functional and molecular characteristics of the healthy human alveolar epithelium, we have developed a new method to immortalise primary human alveolar epithelial lung cells using a non-viral vector to transfect the telomerase catalytic subunit (hTERT) and the simian virus 40 large-tumour antigen (SV40). Twelve strains of immortalised cells (ICs) were generated and characterised using molecular, immunochemical and morphological techniques. Cell proliferation and sensitivity to polystyrene nanoparticles (PS) were evaluated. ICs expressed caveolin-1, podoplanin and receptor for advanced glycation end-products (RAGE), and most cells were negative for alkaline phosphatase staining, indicating characteristics of AT1-like cells. However, most strains also contained some cells that expressed pro-surfactant protein C, classically described to be expressed only by AT2 cells. Thus, the ICs mimic the cellular heterogeneity in the human alveolar epithelium. These ICs can be passaged, replicate rapidly and remain confluent beyond 15 days. ICs showed differential sensitivity to positive and negatively charged PS nanoparticles, illustrating their potential value as an in vitro model to study respiratory bioreactivity. These novel ICs offer a unique resource to study human alveolar epithelial biology.
AU  - Katsumiti,A
AU  - Ruenraroengsak,P
AU  - Cajaraville,MP
AU  - Thorley,AJ
AU  - Tetley,T
DO  - 10.1038/s41598-020-77191-y
PY  - 2020///
SN  - 2045-2322
TI  - Immortalisation of primary human alveolar epithelial cells using a non-viral vector to study respiratory bioreactivity in vitro
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-020-77191-y
UR  - http://hdl.handle.net/10044/1/85095
VL  - 10
ER  -
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