Imperial College London
Alternate

ProfessorZoltanTakats

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Analytical Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 2760z.takats

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

228 results found

Takats Z, Wiseman JM, Ifa DR, Cooks RGet al., 2008, In Situ Desorption Electrospray Ionization (DESI) Analysis of Tissue Sections., CSH Protoc, Vol: 2008

INTRODUCTIONDesorption electrospray ionization (DESI) allows in situ analysis of biological tissues. The analysis of less abundant protein constituents within a tissue sample often requires the removal of lipid species prior to analysis, similar to the situation with matrix-assisted laser desorption/ionization (MALDI). After removal of lipid constituents, the tissue can be treated with protease to degrade proteins present in the tissue. The tryptic products can be investigated directly from the tissue using DESI. The spectra obtained feature ions of tryptic fragments from abundant proteins present in the tissue sample. The digestion is usually not complete; hence, the presence of missed cleavage sites is typical in the peptides detected. The signal is more stable for longer times than in the case of deposited samples, so the recording of mass spectrometry (MS)/MS data is simple in this case. DESI-MS is an emerging technique with great promise, but its application range is still being investigated. Therefore, the protocol for DESI-MS analysis of tissue sections presented here provides general procedures used for the applications that have been investigated so far. Optimal ion source parameters and surface types may vary depending on the application.

Journal article

Takats Z, Wiseman JM, Ifa DR, Cooks RGet al., 2008, Desorption Electrospray Ionization: Proteomics Studies by a Method That Bridges ESI and MALDI., CSH Protoc, Vol: 2008

INTRODUCTIONDesorption electrospray ionization (DESI) is a desorption ionization (DI) method by nature, and, like matrix-assisted laser desorption/ionization (MALDI), it is used for the analysis of material present on a surface. DESI includes features reminiscent of electrospray ionization (ESI) in respect to both its instrumental and mechanistic aspects. However, the analyte in the DESI experiment is not in solution as in ESI. Instead, a microelectrospray ion source is used to produce charged droplets, ionic clusters, and/or gas-phase ions (depending on chosen experimental conditions), and these are directed at the sample surface. The sample is present in the ambient environment. An electrical potential of several kilovolts (kV) is applied to the spray solution, and pneumatic nebulization is used to assist in desolvation. Ionization of molecules present on the sample surface occurs upon the impact of the ESI-originated, charged particles with the surface. Surfaces include deposited samples on sample holder targets as well as surfaces of natural objects such as biological tissues or minerals.

Journal article

Takats Z, Wiseman JM, Ifa DR, Cooks RGet al., 2008, Desorption Electrospray Ionization (DESI) Analysis of Intact Proteins/Oligopeptides., CSH Protoc, Vol: 2008

INTRODUCTIONDesorption electrospray ionization (DESI) is amenable to the study of intact proteins in complex mixtures, including blood or other biological media. Intact proteins can be desorbed and ionized from the surface under gentle (soft) conditions to produce compact conformations of the protein. A procedure for DESI analysis of intact proteins and oligopeptides using mass spectrometry (MS) is described here. DESI-MS is an emerging technique with great promise, but its application range is still being investigated. Therefore, the protocol presented here provides general procedures used for the applications that have been investigated so far. Optimal ion source parameters and surface types may vary depending on the application.

Journal article

Takats Z, Wiseman JM, Ifa DR, Cooks RGet al., 2008, Desorption Electrospray Ionization (DESI) Analysis of Tryptic Digests/Peptides., CSH Protoc, Vol: 2008

INTRODUCTIONThe analytical utility of desorption electrospray ionization (DESI) is such that it can be applied to qualitative proteomics research in the same way as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) methods, although little work has yet been reported in this regard. Because DESI is a surface analysis technique and easily automated, it can be implemented for high-throughput applications, which include the analysis of chromatographic fractions of digested proteins. The analysis of tryptic peptides follows the same protocols as in typical MALDI or ESI methods, except that the mixture is spotted directly onto an insulating surface, allowed to dry, and analyzed directly without adding matrix compounds (as in the case of MALDI methods). The spectral characteristics are similar to those of ESI in that both singly and multiply charged analyte ions are detected. Spectra are highly similar to electrospray spectra of tryptic digests with regard to the overwhelming presence of multiply charged ions of peptides. DESI-mass spectrometry (DESI-MS) is an emerging technique with great promise, but its application range is still being investigated. Therefore, the protocol for DESI-MS analysis of tryptic digests/peptides presented here provides general procedures used for the applications that have been investigated so far. Optimal ion source parameters and surface types may vary, depending on the application.

Journal article

Taban LM, van der Burgt YEM, Duursma M, Takats Z, Seynen M, Konijnenburg M, Vijftigschild A, Attema I, Heeren RMAet al., 2008, A novel workflow control system for Fourier transform ion cyclotron resonance mass spectrometry allows for unique on-the-fly data-dependent decisions, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol: 22, Pages: 1245-1256, ISSN: 0951-4198

Journal article

Takats Z, Kobliha V, Sevcik K, Novak P, Kruppa G, Lemr K, Havlicek Vet al., 2008, Characterization of DESI-FTICR mass spectrometry - from ECD to accurate mass tissue analysis, JOURNAL OF MASS SPECTROMETRY, Vol: 43, Pages: 196-203, ISSN: 1076-5174

Journal article

Vargha M, Takats Z, Konopka A, Nakatsu CHet al., 2006, Optimization of MALDI-TOF MS for strain level differentiation of <i>Arthrobacter</i> isolates, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 66, Pages: 399-409, ISSN: 0167-7012

Journal article

Cooks RG, Ouyang Z, Takats Z, Wiseman JMet al., 2006, Ambient mass spectrometry, SCIENCE, Vol: 311, Pages: 1566-1570, ISSN: 0036-8075

Journal article

Myung S, Wiseman JM, Valentine SJ, Takáts Z, Cooks RG, Clemmer DEet al., 2006, Coupling desorption electrospray ionization with ion mobility/mass spectrometry for analysis of protein structure:: Evidence for desorption of folded and denatured states, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 110, Pages: 5045-5051, ISSN: 1520-6106

Journal article

Chen HW, Talaty NN, Takáts Z, Cooks RGet al., 2005, Desorption electrospray ionization mass spectrometry for high-throughput analysis of pharmaceutical samples in the ambient environment, ANALYTICAL CHEMISTRY, Vol: 77, Pages: 6915-6927, ISSN: 0003-2700

Journal article

Cotte-Rodríguez I, Takáts Z, Talaty N, Chen HW, Cooks RGet al., 2005, Desorption electrospray ionization of explosives on surfaces:: Sensitivity and selectivity enhancement by reactive desorption electrospray ionization, ANALYTICAL CHEMISTRY, Vol: 77, Pages: 6755-6764, ISSN: 0003-2700

Journal article

Cotte-Rodríguez I, Takáts Z, Talaty N, Chen H, Cooks RGet al., 2005, Desorption electrospray ionization of explosives on surfaces: sensitivity and selectivity enhancement by reactive desorption electrospray ionization., Anal Chem, Vol: 77, Pages: 6755-6764, ISSN: 0003-2700

Desorption electrospray ionization (DESI), an ambient mass spectrometry technique, is used for trace detection of the explosives trinitrohexahydro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), 2,4,6-trinitrotoluene (TNT), Pentaerythritol tetranitrate (PETN), and their plastic compositions (Composition C-4, Semtex-H, Detasheet) directly from a wide variety of surfaces (metal, plastic, paper, polymer) without sample preparation or pretreatment. Analysis of the explosives is performed under ambient conditions from virtually any surface in very short times (<5 s) including confirmatory tandem mass spectrometry (MS/MS) experiments, while retaining the sensitivity and specificity that mass spectrometry offers. Increased selectivity is obtained both by MS/MS and by performing additional experiments in which additives are included in the spray solvent. These reactive DESI experiments (reactions accompanying desorption) produce such ions as the chloride and trifluoroacetate adducts of RDX and HMX or the Meisenheimer complex of TNT. Desorption atmospheric pressure chemical ionization, a variant of DESI that uses gas-phase ions generated by atmospheric pressure corona discharges of toluene or other organic compounds, provides evidence for a heterogeneous-phase (gaseous ion/absorbed analyte) charge-transfer mechanism of DESI ionization in the case of explosives. Plastic explosives on surfaces were analyzed directly as fingerprints, without sample preparation, to test DESI as a possible method for in situ detection of explosives-contaminated surfaces. DESI also allowed detection of explosives in complex matrixes, including lubricants, household cleaners, vinegar, and diesel fuel. Absolute limits of detection for the neat explosives were subnanogram in all cases and subpicogram in the case of TNT. The DESI response was linear over 3 orders of magnitude for TNT. Quantification of RDX on paper gave a precision (RSD) of 2.3%. Pure water could be us

Journal article

Takáts Z, Wiseman JM, Cooks RG, 2005, Ambient mass spectrometry using desorption electrospray ionization (DESI):: instrumentation, mechanisms and applications in forensics, chemistry, and biology, JOURNAL OF MASS SPECTROMETRY, Vol: 40, Pages: 1261-1275, ISSN: 1076-5174

Journal article

Takáts Z, Cotte-Rodriguez I, Talaty N, Chen HW, Cooks RGet al., 2005, Direct, trace level detection of explosives on ambient surfaces by desorption electrospray ionization mass spectrometry, CHEMICAL COMMUNICATIONS, Pages: 1950-1952, ISSN: 1359-7345

Journal article

Vargha M, Takáts Z, Márialigeti K, 2005, Degradation of atrazine in a laboratory scale model system with Danube river sediment, WATER RESEARCH, Vol: 39, Pages: 1560-1568, ISSN: 0043-1354

Journal article

Misharin AS, Laughlin BC, Vilkov A, Takáts Z, Zheng OY, Cooks RGet al., 2005, High-throughput mass spectrometer using atmospheric pressure ionization and a cylindrical ion trap array, ANALYTICAL CHEMISTRY, Vol: 77, Pages: 459-470, ISSN: 0003-2700

Journal article

Talaty N, Takáts Z, Cooks RG, 2005, Rapid <i>in situ</i> detection of alkaloids in plant tissue under ambient conditions using desorption electrospray ionization, ANALYST, Vol: 130, Pages: 1624-1633, ISSN: 0003-2654

Journal article

Wiseman JM, Takáts Z, Gologan B, Davisson VJ, Cooks RGet al., 2005, Direct characterization of enzyme-substrate complexes by using electrosonic spray ionization mass spectrometry, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 44, Pages: 913-916, ISSN: 1433-7851

Journal article

Wiseman JM, Puolitaival SM, Takáts Z, Cooks RG, Caprioli RMet al., 2005, Mass spectrometric profiling of intact biological tissue by using desorption electrospray ionization, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 44, Pages: 7094-7097, ISSN: 1433-7851

Journal article

Gologan B, Takáts Z, Alvarez J, Wiseman JM, Talaty N, Ouyang Z, Cooks RGet al., 2004, Ion soft-landing into liquids:: Protein identification, separation, and purification with retention of biological activity, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol: 15, Pages: 1874-1884, ISSN: 1044-0305

Journal article

Blake TA, Zheng OY, Wiseman JM, Takáts Z, Guymon AJ, Kothari S, Cooks RGet al., 2004, Preparative linear ion trap mass spectrometer for separation and collection of purified proteins and peptides in arrays using ion soft landing, ANALYTICAL CHEMISTRY, Vol: 76, Pages: 6293-6305, ISSN: 0003-2700

Journal article

Takáts Z, Wiseman JM, Gologan B, Cooks RGet al., 2004, Mass spectrometry sampling under ambient conditions with desorption electrospray ionization, SCIENCE, Vol: 306, Pages: 471-473, ISSN: 0036-8075

Journal article

Nanita SC, Takats Z, Cooks RG, Myung S, Clemmer DEet al., 2004, Chiral enrichment of serine via formation, dissociation, and soft-landing of octameric cluster ions, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol: 15, Pages: 1360-1365, ISSN: 1044-0305

Journal article

Takáts Z, Wiseman JM, Gologan B, Cooks RGet al., 2004, Electrosonic spray ionization.: A gentle technique for generating folded proteins and protein complexes in the gas phase and for studying ion -: Molecule reactions at atmospheric pressure, ANALYTICAL CHEMISTRY, Vol: 76, Pages: 4050-4058, ISSN: 0003-2700

Journal article

Takáts Z, Cooks RG, 2004, Thermal formation of serine octamer ions, CHEMICAL COMMUNICATIONS, Pages: 444-445, ISSN: 1359-7345

Journal article

Nagy K, Pollreisz F, Takáts Z, Vékey Ket al., 2004, Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol: 18, Pages: 2473-2478, ISSN: 0951-4198

Journal article

Schlosser G, Takáts Z, Vékey K, 2003, Formation of solvated ions in the atmospheric interface of an electrospray ionization triple-quadrupole mass spectrometer, JOURNAL OF MASS SPECTROMETRY, Vol: 38, Pages: 1245-1251, ISSN: 1076-5174

Journal article

Takats Z, Nanita SC, Schlosser G, Vekey K, Cooks RGet al., 2003, Atmospheric pressure gas-phase H/D exchange of serine octamers, ANALYTICAL CHEMISTRY, Vol: 75, Pages: 6147-6154, ISSN: 0003-2700

Journal article

Ouyang Z, Takáts Z, Blake TA, Gologan B, Guymon AJ, Wiseman JM, Oliver JC, Davisson VJ, Cooks RGet al., 2003, Preparing protein microarrays by soft-landing of mass-selected ions, SCIENCE, Vol: 301, Pages: 1351-1354, ISSN: 0036-8075

Journal article

Takáts Z, Schlosser G, Vékey K, 2003, Hydrogen/deuterium exchange of electrosprayed ions in the atmospheric interface of a commercial triple-quadrupole mass spectrometer, Symposium in Honor of Helmut Schwaz, Publisher: ELSEVIER SCIENCE BV, Pages: 729-741, ISSN: 1387-3806

Conference paper

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