Imperial College London

ProfessorAdnanCustovic

Faculty of MedicineNational Heart & Lung Institute

Professor of Paediatric Allergy
 
 
 
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a.custovic CV

 
 
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Location

 

220Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bahri:2018:10.1016/j.jaci.2018.01.043,
author = {Bahri, R and Custovic, A and Korosec, P and Tsoumani, M and Barron, M and Wu, J and Sayers, R and Weimann, A and Ruiz, Garcia M and Patel, N and Robb, A and Shamji, M and Fontanella, S and Silar, M and Mills, C and Simpson, A and Turner, PJ and Bulfone-Paus, S},
doi = {10.1016/j.jaci.2018.01.043},
journal = {Journal of Allergy and Clinical Immunology},
pages = {485--496.e16},
title = {Mast cell activation test in the diagnosis of allergic disease and anaphylaxis},
url = {http://dx.doi.org/10.1016/j.jaci.2018.01.043},
volume = {142},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - BackgroundFood allergy is an increasing public health issue and the most common cause of life-threatening anaphylactic reactions. Conventional allergy tests assess for the presence of allergen-specific IgE, significantly overestimating the rate of true clinical allergy and resulting in overdiagnosis and adverse effect on health-related quality of life.ObjectiveTo undertake initial validation and assessment of a novel diagnostic tool, we used the mast cell activation test (MAT).MethodsPrimary human blood-derived mast cells (MCs) were generated from peripheral blood precursors, sensitized with patients' sera, and then incubated with allergen. MC degranulation was assessed by means of flow cytometry and mediator release. We compared the diagnostic performance of MATs with that of existing diagnostic tools to assess in a cohort of peanut-sensitized subjects undergoing double-blind, placebo-controlled challenge.ResultsHuman blood-derived MCs sensitized with sera from patients with peanut, grass pollen, and Hymenoptera (wasp venom) allergy demonstrated allergen-specific and dose-dependent degranulation, as determined based on both expression of surface activation markers (CD63 and CD107a) and functional assays (prostaglandin D2 and β-hexosaminidase release). In this cohort of peanut-sensitized subjects, the MAT was found to have superior discrimination performance compared with other testing modalities, including component-resolved diagnostics and basophil activation tests. Using functional principle component analysis, we identified 5 clusters or patterns of reactivity in the resulting dose-response curves, which at preliminary analysis corresponded to the reaction phenotypes seen at challenge.ConclusionThe MAT is a robust tool that can confer superior diagnostic performance compared with existing allergy diagnostics and might be useful to explore differences in effector cell function between basophils and MCs during allergic reactions.
AU - Bahri,R
AU - Custovic,A
AU - Korosec,P
AU - Tsoumani,M
AU - Barron,M
AU - Wu,J
AU - Sayers,R
AU - Weimann,A
AU - Ruiz,Garcia M
AU - Patel,N
AU - Robb,A
AU - Shamji,M
AU - Fontanella,S
AU - Silar,M
AU - Mills,C
AU - Simpson,A
AU - Turner,PJ
AU - Bulfone-Paus,S
DO - 10.1016/j.jaci.2018.01.043
EP - 496
PY - 2018///
SN - 0091-6749
SP - 485
TI - Mast cell activation test in the diagnosis of allergic disease and anaphylaxis
T2 - Journal of Allergy and Clinical Immunology
UR - http://dx.doi.org/10.1016/j.jaci.2018.01.043
UR - http://hdl.handle.net/10044/1/56335
VL - 142
ER -