Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sivaneson:2011:10.1111/j.1365-2958.2010.07527.x,
author = {Sivaneson, M and Mikkelsen, H and Ventre, I and Bordi, C and Filloux, A},
doi = {10.1111/j.1365-2958.2010.07527.x},
journal = {Molecular Microbiology},
pages = {1353--1366},
title = {Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression},
url = {http://dx.doi.org/10.1111/j.1365-2958.2010.07527.x},
volume = {79},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Pseudomonas aeruginosa is responsible for chronic and acute infections in humans. Chronic infections are associated with production of fimbriae and the formation of a biofilm. The twocomponent system Roc1 is named after its role in the regulation of cup genes, which encode components of a machinery allowing assembly of fimbriae. A noncharacterized gene cluster, roc2, encodes components homologous to the Roc1 system. We show that crossregulation occurs between the Roc1 and Roc2 signalling pathways. We demonstrate that the sensors RocS2 and RocS1 converge on the response regulator RocA1 to control cupC gene expression. This control is independent of the response regulator RocA2. Instead, we show that these sensors act via the RocA2 response regulator to repress the mexABoprM genes. These genes encode a multidrug efflux pump and are upregulated in the rocA2 mutant, which is less susceptible to antibiotics. It has been reported that in cystic fibrosis lungs, in which P. aeruginosa adopts the biofilm lifestyle, most isolates have an inactive MexABOprM pump. The concomitant RocS2dependent upregulation of cupC genes (biofilm formation) and downregulation of mexABoprM genes (antibiotic resistance) is in agreement with this observation. It suggests that the Roc systems may sense the environment in the cystic fibrosis lung.
AU  - Sivaneson,M
AU  - Mikkelsen,H
AU  - Ventre,I
AU  - Bordi,C
AU  - Filloux,A
DO  - 10.1111/j.1365-2958.2010.07527.x
EP  - 1366
PY  - 2011///
SN  - 0950-382X
SP  - 1353
TI  - Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/j.1365-2958.2010.07527.x
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287589000020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/65709
VL  - 79
ER  -
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