Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mikkelsen:2013:10.1111/mmi.12287,
author = {Mikkelsen, H and Hui, K and Barraud, N and Filloux, A},
doi = {10.1111/mmi.12287},
journal = {Molecular Microbiology},
pages = {450--463},
title = {The pathogenicity island encoded PvrSR/RcsCB regulatory network controls biofilm formation and dispersal in Pseudomonas aeruginosa PA14},
url = {http://dx.doi.org/10.1111/mmi.12287},
volume = {89},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Pseudomonas aeruginosa biofilm formation is linked to persistent infections in humans. Biofilm formation is facilitated by extracellular appendages, some of which are assembled by the Chaperone Usher Pathway (Cup). The cupD gene cluster is located on the PAPI1 pathogenicity island of strain PA14 and has probably been acquired together with four genes encoding twocomponent signal transduction proteins. We have previously showed that the RcsB response regulator activates expression of the cupD genes, which leads to the production of CupD fimbriae and increased attachment. Here we show that RcsB activity is tightly modulated by two sensors, RcsC and PvrS. While PvrS acts as a kinase that enhances RcsB activity, RcsC has a dual function, first as a phosphorelay, and second as a phosphatase. We found that, under certain growth conditions, overexpression of RcsB readily induces biofilm dispersal. Microarray analysis shows that RcsB positively controls expression of pvrR that encodes the phosphodiesterase required for this dispersal process. Finally, in addition to the PAPI1 encoded cupD genes, RcsB controls several genes on the core genome, some of which encode orphan response regulators. We thus discovered that RcsB is central to a large regulatory network that finetunes the switch between biofilm formation and dispersal.
AU  - Mikkelsen,H
AU  - Hui,K
AU  - Barraud,N
AU  - Filloux,A
DO  - 10.1111/mmi.12287
EP  - 463
PY  - 2013///
SN  - 0950-382X
SP  - 450
TI  - The pathogenicity island encoded PvrSR/RcsCB regulatory network controls biofilm formation and dispersal in Pseudomonas aeruginosa PA14
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.12287
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322332800004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/65714
VL  - 89
ER  -
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