Imperial College London
Alternate

ProfessorAlexandraPorter

Faculty of Engineering, Department of Materials

Professor of Bio-imaging and Analysis
 
 
 
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Contact

 

+44 (0)20 7594 9691a.porter

 
 
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Location

 

B341 Royal School of MinesRoyal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Greaves:2023:10.1038/s42003-023-04943-7,
author = {Greaves, G and Kiryushko, D and Auner, H and Porter, A and Phillips, C},
doi = {10.1038/s42003-023-04943-7},
journal = {Communications Biology},
pages = {1--7},
title = {Label-free nanoscale mapping of intracellular organelle chemistry},
url = {http://dx.doi.org/10.1038/s42003-023-04943-7},
volume = {6},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The ability to image cell chemistry at the nanoscale is key for understanding cell biology, but many optical microscopies are restricted by the ~(200–250)nm diffraction limit. Electron microscopy and super-resolution fluorescence techniques beat this limit, but rely on staining and specialised labelling to generate image contrast. It is challenging, therefore, to obtain information about the functional chemistry of intracellular components. Here we demonstrate a technique for intracellular label-free chemical mapping with nanoscale (~30 nm) resolution. We use a probe-based optical microscope illuminated with a mid-infrared laser whose wavelengths excite vibrational modes of functional groups occurring within biological molecules. As a demonstration, we chemically map intracellular structures in human multiple myeloma cells and compare the morphologies with electron micrographs of the same cell line. We also demonstrate label-free mapping at wavelengths chosen to target the chemical signatures of proteins and nucleic acids, in a way that can be used to identify biochemical markers in the study of disease and pharmacology.
AU  - Greaves,G
AU  - Kiryushko,D
AU  - Auner,H
AU  - Porter,A
AU  - Phillips,C
DO  - 10.1038/s42003-023-04943-7
EP  - 7
PY  - 2023///
SN  - 2399-3642
SP  - 1
TI  - Label-free nanoscale mapping of intracellular organelle chemistry
T2  - Communications Biology
UR  - http://dx.doi.org/10.1038/s42003-023-04943-7
UR  - https://www.nature.com/articles/s42003-023-04943-7
UR  - http://hdl.handle.net/10044/1/104566
VL  - 6
ER  -
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