Imperial College London
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DrAndreaWeisse

Faculty of Medicine, Department of Infectious Disease

Honorary Lecturer
 
 
 
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Contact

 

andrea.weisse

 
 
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Location

 

Huxley BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Nikolados:2021:10.1007/978-1-0716-1032-9_13,
author = {Nikolados, E-M and Weiße, AY and Oyarzún, DA},
booktitle = {Methods in Molecular Biology},
doi = {10.1007/978-1-0716-1032-9_13},
pages = {267--291},
publisher = {Springer},
title = {Prediction of cellular burden with host-circuit models.},
url = {http://dx.doi.org/10.1007/978-1-0716-1032-9_13},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host-circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.
AU  - Nikolados,E-M
AU  - Weiße,AY
AU  - Oyarzún,DA
DO  - 10.1007/978-1-0716-1032-9_13
EP  - 291
PB  - Springer
PY  - 2021///
SP  - 267
TI  - Prediction of cellular burden with host-circuit models.
T1  - Methods in Molecular Biology
UR  - http://dx.doi.org/10.1007/978-1-0716-1032-9_13
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33405227
UR  - https://link.springer.com/protocol/10.1007%2F978-1-0716-1032-9_13
UR  - http://hdl.handle.net/10044/1/91964
ER  -
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