Imperial College London
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DrAnitaHall

Faculty of Natural Sciences, Department of Life Sciences

Senior Teaching Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5193anita.hall Website

 
 
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Location

 

206Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bonilla:2008,
author = {Bonilla, S and Hall, AC and Pinto, L and Attardo, A and Gotz, M and Huttner, WB and Arenas, E},
journal = {Glia},
pages = {809--820},
title = {Identification of midbrain floor plate radial glia-like cells as dopaminergic progenitors.},
volume = {56},
year = {2008}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The floor plate (FP), a signaling center and a structure rich in radial glia-like cells, has been traditionally thought to be devoid of neurons and neuronal progenitors. However, in the midbrain, the FP contains neurons of the dopaminergic (DA) lineage that require contact with radial glia-like cells for their induction. We, therefore, decided to explore the interaction relationship between radial glia and neurons during DA neurogenesis. Taking advantage of a novel FP radial glia-like cell culture system and retroviruses, DA neurons were lineage traced in vitro. In utero BrdU pulse-chases extensively labeled the midbrain FP and traced DA neurons both in vivo and in FP cultures. Moreover, from E9.5 to E13.5 the midbrain FP contained dividing cells only in the most apical part of the neuroepithelium, in cells identified as radial glia-like cells. We, therefore, hypothesized that midbrain FP radial glia-like cells could be DA progenitors and tested our hypothesis in vivo. Lineage tracing of DA progenitors with EGFP in Tis21-EGFP knock-in mice, and genetic fate mapping in GLAST::CreERT2/ZEG mice identified the neuroepithelium of the midbrain FP, and specifically, GLAST+ radial glia-like cells as DA progenitors. Combined, our experiments support the concept that the midbrain FP differs from other FP regions and demonstrate that FP radial glia-like cells in the midbrain are neurogenic and give rise to midbrain DA neurons.
AU  - Bonilla,S
AU  - Hall,AC
AU  - Pinto,L
AU  - Attardo,A
AU  - Gotz,M
AU  - Huttner,WB
AU  - Arenas,E
EP  - 820
PY  - 2008///
SP  - 809
TI  - Identification of midbrain floor plate radial glia-like cells as dopaminergic progenitors.
T2  - Glia
VL  - 56
ER  -
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