Imperial College London
Alternate

ProfessorCharlesBangham

Institute of Infection

Co-Director of the Institute of Infection
 
 
 
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Contact

 

+44 (0)20 7594 3730c.bangham Website

 
 
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Assistant

 

Ms Linda Hollick +44 (0)20 7594 3729

 
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Location

 

115Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kirk:2011:10.1186/1742-4690-8-81,
author = {Kirk, PDW and Witkover, A and Courtney, A and Lewin, AM and Wait, R and Stumpf, MPH and Richardson, S and Taylor, GP and Bangham, CRM},
doi = {10.1186/1742-4690-8-81},
journal = {Retrovirology},
title = {Plasma proteome analysis in HTLV-1-associated myelopathy/tropical spastic paraparesis},
url = {http://dx.doi.org/10.1186/1742-4690-8-81},
volume = {8},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background:  Human T lymphotropic virus Type 1 (HTLV-1) causes a chronic inflammatory disease of the central nervous system known as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM) which resembles chronic spinal forms of multiple sclerosis (MS).  The pathogenesis of HAM remains uncertain.  To aid in the differential diagnosis of HAM and to identify pathogenetic mechanisms we analysed the plasma proteome in asymptomatic HTLV-1 carriers (ACs), patients with HAM, uninfected controls and patients with MS.  We used surface-enhanced laser desorption-ionization (SELDI) mass spectrometry to analyse the plasma proteome in 68 HTLV-1-infected individuals (in two non-overlapping sets, each comprising 17 patients with HAM and 17 ACs), 16 uninfected controls, and 11 patients with secondary progressive MS.  Candidate biomarkers were identified by tandem Q-TOF mass spectrometry. Results:  The concentrations of three plasma proteins – high [2-microglobulin], high [Calgranulin B], and low [apolipoprotein A2] – were specifically associated with HAM, independently of proviral load.  The plasma [2-microglobulin] was positively correlated with disease severity. Conclusions:  The results indicate that monocytes are activated by contact with activated endothelium in HAM.  Using 2-microglobulin and Calgranulin B alone we derive a diagnostic algorithm that correctly classified the disease status (presence or absence of HAM) in 81% of HTLV-1-infected subjects in the cohort.
AU  - Kirk,PDW
AU  - Witkover,A
AU  - Courtney,A
AU  - Lewin,AM
AU  - Wait,R
AU  - Stumpf,MPH
AU  - Richardson,S
AU  - Taylor,GP
AU  - Bangham,CRM
DO  - 10.1186/1742-4690-8-81
PY  - 2011///
SN  - 1742-4690
TI  - Plasma proteome analysis in HTLV-1-associated myelopathy/tropical spastic paraparesis
T2  - Retrovirology
UR  - http://dx.doi.org/10.1186/1742-4690-8-81
UR  - http://hdl.handle.net/10044/1/27412
VL  - 8
ER  -
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