Imperial College London
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Professor Cleo Kontoravdi

Faculty of Engineering, Department of Chemical Engineering

Professor of Biological Systems Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6655cleo.kontoravdi98 Website

 
 
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Location

 

310ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sachio:2024:10.1016/j.chroma.2024.464890,
author = {Sachio, S and Likozar, B and Kontoravdi, C and Papathanasiou, MM},
doi = {10.1016/j.chroma.2024.464890},
journal = {J Chromatogr A},
title = {Computer-aided design space identification for screening of protein A affinity chromatography resins.},
url = {http://dx.doi.org/10.1016/j.chroma.2024.464890},
volume = {1722},
year = {2024}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The rapidly growing market of monoclonal antibodies (mAbs) within the biopharmaceutical industry has incentivised numerous works on the design of more efficient production processes. Protein A affinity chromatography is regarded as one of the best processes for the capture of mAbs. Although the screening of Protein A resins has been previously examined, process flexibility has not been considered to date. Examining performance alongside flexibility is crucial for the design of processes that can handle disturbances arising from the feed stream. In this work, we present a model-based approach for the identification of design spaces, enhanced by machine learning. We demonstrate its capabilities on the design of a Protein A chromatography unit, screening five industrially relevant resins. The computational results favourably compare to experimental data and a resin performance comparison is presented. An improvement on the computational time by a factor of 300,000 is achieved using the machine learning aided methodology. This allowed for the identification of 5,120 different design spaces in only 19 h.
AU  - Sachio,S
AU  - Likozar,B
AU  - Kontoravdi,C
AU  - Papathanasiou,MM
DO  - 10.1016/j.chroma.2024.464890
PY  - 2024///
TI  - Computer-aided design space identification for screening of protein A affinity chromatography resins.
T2  - J Chromatogr A
UR  - http://dx.doi.org/10.1016/j.chroma.2024.464890
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38598892
VL  - 1722
ER  -
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