Imperial College London
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ProfessorDorianHaskard

Faculty of Medicine, National Heart & Lung Institute

Proconsul & Professor Cardiovascular Medicine & Rheumatology
 
 
 
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Contact

 

+44 (0)7725 159 719d.haskard Website

 
 
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Location

 

540ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hartley:2022:10.3390/antiox11020271,
author = {Hartley, A and Pradeep, M and Van, den Berg V and Khan, AHA and Shah, HA and Allaf, M and Chow, A and Caga-Anan, M and Shalhoub, J and Koenig, W and Fisher, M and Haskard, D and Khamis, R and Hartley, A and Khamis, R and Shalhoub, J and Haskard, D},
doi = {10.3390/antiox11020271},
journal = {Antioxidants},
title = {Depletion of homeostatic antibodies against malondialdehyde-modified low-density lipoprotein correlates with adverse events in major vascular surgery},
url = {http://dx.doi.org/10.3390/antiox11020271},
volume = {11},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We aimed to investigate if major vascular surgery induces LDL oxidation, and whether circulating antibodies against malondialdehyde-modified LDL (MDA-LDL) alter dynamically in this setting. We also questioned relationships between these biomarkers and post-operative cardiovascular events. Major surgery can induce an oxidative stress response. However, the role of the humoral immune system in clearance of oxidized LDL following such an insult is unknown. Plasma samples were obtained from a prospective cohort of 131 patients undergoing major non-cardiac vascular surgery, with samples obtained preoperatively and at 24- and 72 h postoperatively. Enzyme-linked immunoassays were developed to assess MDA-LDL-related antibodies and complexes. Adverse events were myocardial infarction (primary outcome), and a composite of unstable angina, stroke and all-cause mortality (secondary outcome). MDA-LDL significantly increased at 24 h post-operatively (p < 0.0001). Conversely, levels of IgG and IgM anti-MDA-LDL, as well as IgG/IgM-MDA-LDL complexes and total IgG/IgM, were significantly lower at 24 h (each p < 0.0001). A smaller decrease in IgG anti-MDA-LDL related to combined clinical adverse events in a post hoc analysis, withstanding adjustment for age, sex, and total IgG (OR 0.13, 95% CI [0.03–0.5], p < 0.001; p value for trend <0.001). Major vascular surgery resulted in an increase in plasma MDA-LDL, in parallel with a decrease in antibody/complex levels, likely due to antibody binding and subsequent removal from the circulation. Our study provides novel insight into the role of the immune system during the oxidative stress of major surgery, and suggests a homeostatic clearance role for IgG antibodies, with greater reduction relating to downstream adverse events.
AU  - Hartley,A
AU  - Pradeep,M
AU  - Van,den Berg V
AU  - Khan,AHA
AU  - Shah,HA
AU  - Allaf,M
AU  - Chow,A
AU  - Caga-Anan,M
AU  - Shalhoub,J
AU  - Koenig,W
AU  - Fisher,M
AU  - Haskard,D
AU  - Khamis,R
AU  - Hartley,A
AU  - Khamis,R
AU  - Shalhoub,J
AU  - Haskard,D
DO  - 10.3390/antiox11020271
PY  - 2022///
SN  - 2076-3921
TI  - Depletion of homeostatic antibodies against malondialdehyde-modified low-density lipoprotein correlates with adverse events in major vascular surgery
T2  - Antioxidants
UR  - http://dx.doi.org/10.3390/antiox11020271
UR  - http://hdl.handle.net/10044/1/94619
VL  - 11
ER  -
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