Imperial College London
Alternate

Professor Daqing Ma, MD, PhD

Faculty of Medicine, Department of Surgery & Cancer

Professor of Anaesthesia
 
 
 
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Contact

 

+44 (0)20 3315 8495d.ma Website

 
 
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Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
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Location

 

G3.44Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhu:2024:10.7150/thno.89592,
author = {Zhu, J and Chen, C and Liu, X and He, M and Fang, Y and Wang, L and Jia, J and Guo, J and Zhao, Z and Gao, C and He, J and Xu, C and Xu, F and Ma, D and Wang, J and Zhang, Z},
doi = {10.7150/thno.89592},
journal = {Theranostics},
pages = {480--495},
title = {Cerebellar Purkinje cell firing promotes conscious recovery from anesthesia state through coordinating neuronal communications with motor cortex.},
url = {http://dx.doi.org/10.7150/thno.89592},
volume = {14},
year = {2024}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: The neurobiological basis of gaining consciousness from unconscious state induced by anesthetics remains unknown. This study was designed to investigate the involvement of the cerebello-thalamus-motor cortical loop mediating consciousness transitions from the loss of consciousness (LOC) induced by an inhalational anesthetic sevoflurane in mice. Methods: The neural tracing and fMRI together with opto-chemogenetic manipulation were used to investigate the potential link among cerebello-thalamus-motor cortical brain regions. The fiber photometry of calcium and neurotransmitters, including glutamate (Glu), γ-aminobutyric acid (GABA) and norepinephrine (NE), were monitored from the motor cortex (M1) and the 5th lobule of the cerebellar vermis (5Cb) during unconsciousness induced by sevoflurane and gaining consciousness after sevoflurane exposure. Cerebellar Purkinje cells were optogenetically manipulated to investigate their influence on consciousness transitions during and after sevoflurane exposure. Results: Activation of 5Cb Purkinje cells increased the Ca2+ flux in the M1 CaMKIIα+ neurons, but this increment was significantly reduced by inactivation of posterior and parafascicular thalamic nucleus. The 5Cb and M1 exhibited concerted calcium flux, and glutamate and GABA release during transitions from wakefulness, loss of consciousness, burst suppression to conscious recovery. Ca2+ flux and Glu release in the M1, but not in the 5Cb, showed a strong synchronization with the EEG burst suppression, particularly, in the gamma-band range. In contrast, the Glu, GABA and NE release and Ca2+ oscillations were coherent with the EEG gamma band activity only in the 5Cb during the pre-recovery of consciousness period. The optogenetic activation of Purkinje cells during burst suppression significantly facilitated emergence from anesthesia while the optogenetic inhibition prolonged the time to gaining consciousness. Conclusions: Our data indicate that cerebe
AU  - Zhu,J
AU  - Chen,C
AU  - Liu,X
AU  - He,M
AU  - Fang,Y
AU  - Wang,L
AU  - Jia,J
AU  - Guo,J
AU  - Zhao,Z
AU  - Gao,C
AU  - He,J
AU  - Xu,C
AU  - Xu,F
AU  - Ma,D
AU  - Wang,J
AU  - Zhang,Z
DO  - 10.7150/thno.89592
EP  - 495
PY  - 2024///
SP  - 480
TI  - Cerebellar Purkinje cell firing promotes conscious recovery from anesthesia state through coordinating neuronal communications with motor cortex.
T2  - Theranostics
UR  - http://dx.doi.org/10.7150/thno.89592
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38169536
VL  - 14
ER  -
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