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@article{Jiang:2024:10.12688/f1000research.125877.2,
author = {Jiang, C and Gonzalez-Anton, S and Li, X and Mi, E and Wu, L and Zhao, H and Zhang, G and Lu, A and Lo, Celso C and Ma, D},
doi = {10.12688/f1000research.125877.2},
journal = {F1000Research},
pages = {1491--1491},
title = {General anaesthetics reduce acute lymphoblastic leukaemia malignancies in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms},
url = {http://dx.doi.org/10.12688/f1000research.125877.2},
volume = {11},
year = {2024}
}

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TY  - JOUR
AB  - <ns3:p>Background Acute lymphoblastic leukaemia (ALL) is a common type of cancer in children. General anaesthetics are often used on patients undergoing painful procedures during ALL treatments but their effects on ALL malignancy remain unknown. Herein, we aim to study the effect of propofol and sevoflurane on the migration, homing and chemoresistance of ALL cells. Methods NALM-6 and Reh cells were treated with propofol (5 and 10 μg/ml) or sevoflurane (3.6%) <ns3:italic>in vitro</ns3:italic> for six hours. Then, cells were harvested for adhesion assay and migration assay <ns3:italic>in vitro</ns3:italic>. In <ns3:italic>in vivo</ns3:italic> experiments, GFP-NALM-6 cells were pre-treated with propofol (10 μg/ml) or sevoflurane (3.6%) for six hours. Then, cells were injected intravenously to C57BL/6 female mice followed by intravital microscopy. For chemoresistance study, cells were treated with rising concentrations of Ara-c (0.05-50 nM) plus 10μg/ml of propofol or Ara-C plus 3.6% of sevoflurane for 4 hours, followed by the assessment of cell viability via CCK-8 assay and detection of autophagy via flow cytometry. Results Both anaesthetics reduced <ns3:italic>in vivo</ns3:italic> migration and <ns3:italic>in vivo</ns3:italic> homing as exemplified by 1) the reduction in the number of cells entering the bone marrow and 2) the disturbance in homing location in relation to endosteal surface. Our results indicated that general anaesthetics reduced the surface CXCR4 expression and the adhesion of leukaemia cells to thrombin cleaved osteopontin (OPN) was reduced. Those changes might result in the alterations in migration and homing. In addition, both anaesthetics sensitised ALL cells to Ara-c possibly through CXCR4 mediated mechanisms. Propofol but not sevoflurane enhanced chemo-related cell death via inducing cytotoxic autophagy. Conclusion Together, our data suggest that both propofol and sevoflur
AU  - Jiang,C
AU  - Gonzalez-Anton,S
AU  - Li,X
AU  - Mi,E
AU  - Wu,L
AU  - Zhao,H
AU  - Zhang,G
AU  - Lu,A
AU  - Lo,Celso C
AU  - Ma,D
DO  - 10.12688/f1000research.125877.2
EP  - 1491
PY  - 2024///
SP  - 1491
TI  - General anaesthetics reduce acute lymphoblastic leukaemia malignancies in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms
T2  - F1000Research
UR  - http://dx.doi.org/10.12688/f1000research.125877.2
VL  - 11
ER  -

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Professor Daqing Ma, MD, PhD

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