Imperial College London
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Emeritus ProfessorDesmondSheridan

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
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Contact

 

d.sheridan Website

 
 
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Location

 

C/o C. EnrightGuy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Botchway:2000:10.1046/j.1365-2257.2000.00267.x,
author = {Botchway, AN and Flores, NA and Sheridan, DJ and Cohen, H},
doi = {10.1046/j.1365-2257.2000.00267.x},
journal = {Clin Lab Haematol},
pages = {21--28},
title = {Storage pool defect in pooled buffy coat platelet concentrates within the shelf-life period.},
url = {http://dx.doi.org/10.1046/j.1365-2257.2000.00267.x},
volume = {22},
year = {2000}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Platelet concentrate (PC) transfusions are useful for maintaining haemostasis in a variety of clinical situations. The function of transfused platelets is of critical importance, and changes on storage of buffy coat-prepared PC may influence their haemostatic potential. Total platelet adenine nucleotide content and platelet aggregation responses were studied, serially, in pooled buffy coat-derived PCs (n = 7), stored under UK recommended blood bank conditions, over the stipulated shelf-life of 5 days. Mean platelet volume (MPV), platelet counts and platelet distribution width (PDW) were also quantified. Total platelet ADP content decreased from 4.45+/-0.78 to 3.71+/-0.69 nmol/108 platelets (P<0.01, day 1 versus day 5, mean +/- SEM) over the shelf-life period. This was associated with reduced aggregatory responses: responses (expressed as percentage of maximum height) to 5 and 10 microM ADP decreased from 10.8+/-2.8% to 1.0+/-1.0% (P<0.005, 5 microM, day 1 versus day 5) and from 18.0+/-5.4% to 4.7+/-2.2% (P<0.02, 10 microM, day 1 versus day 5) while the decreased responsiveness was more pronounced for 4 microg/ml of collagen: 49.0+/-13.3% to 7.2+/-7.1% (P<0.01, day 1 versus day 4) and 49.9 +/-13.3% to 2.1+/-1.9% (P<0.001, day 1 versus day 5). These data indicate an acquired storage pool defect that is maximal by day 4 or 5 and accompanied by decreased platelet function, characterized by significant decreases in platelet aggregation responses. Addition of freeze-thawed plasma (autologous day 1) to PCs on days 2, 3, 4 and 5 did not alter the responses to ADP and collagen.
AU  - Botchway,AN
AU  - Flores,NA
AU  - Sheridan,DJ
AU  - Cohen,H
DO  - 10.1046/j.1365-2257.2000.00267.x
EP  - 28
PY  - 2000///
SN  - 0141-9854
SP  - 21
TI  - Storage pool defect in pooled buffy coat platelet concentrates within the shelf-life period.
T2  - Clin Lab Haematol
UR  - http://dx.doi.org/10.1046/j.1365-2257.2000.00267.x
UR  - https://www.ncbi.nlm.nih.gov/pubmed/10762300
VL  - 22
ER  -
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