Imperial College London
Alternate

Prof David C. Stuckey

Faculty of Engineering, Department of Chemical Engineering

Emeritus Professor
 
 
 
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Contact

 

+44 (0)20 7594 5591d.stuckey

 
 
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Location

 

510ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ward:2016:10.1002/jctb.4942,
author = {Ward, K and Cheng, SI and Stuckey, DC},
doi = {10.1002/jctb.4942},
journal = {Journal of Chemical Technology and Biotechnology},
title = {Protein separation using non-ionic and cationic surfactant precipitation},
url = {http://dx.doi.org/10.1002/jctb.4942},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: The predominant use of anionic surfactants to precipitate high isoelectric point (pI) proteins has increased in recent years, simplifying downstream separations. However, few researchers have tested cationic and non-ionic surfactants, whose properties are more desirable. This paper examines the effect of these surfactants on the precipitation efficiency of lysozyme, trypsin inhibitor and bovine serum albumin (BSA). RESULTS: Precipitation of BSA and trypsin inhibitor using the cationics, trioctylmethylammonium chloride (TOMAC) and dimethyl dioctadecyl ammonium chloride (DODMAC) was evaluated, with TOMAC being superior. More than 90% of BSA was precipitated using TOMAC at pH 9.0 with a molar ratio of surfactant/protein (R) of 100:1, while 88% was precipitated using DODMAC. However, for trypsin inhibitor, only 58% was precipitated at an R of 61:1 and pH 6.2 using TOMAC. Protein precipitate recovery using the anionic surfactant sodium bis-[2-ethylhexyl] sulfosuccinate (AOT) was effective only with trypsin inhibitor, with 100% of the protein being recovered. CONCLUSIONS: This study shows the potential of cationics to precipitate low pI proteins, and recover them using the counterionic surfactant AOT, with 100% recovery of trypsin inhibitor. However, non-ionic surfactants were ineffective. The method not only separates, but also preserves protein structure; hence cationic surfactants for low pI protein separation are promising.
AU  - Ward,K
AU  - Cheng,SI
AU  - Stuckey,DC
DO  - 10.1002/jctb.4942
PY  - 2016///
SN  - 1097-4660
TI  - Protein separation using non-ionic and cationic surfactant precipitation
T2  - Journal of Chemical Technology and Biotechnology
UR  - http://dx.doi.org/10.1002/jctb.4942
ER  -
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