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@article{Versi:2023:10.1111/all.15776,
author = {Versi, A and Ivan, FX and Abdel-Aziz, MI and Bates, S and Riley, J and Baribaud, F and Kermani, NZ and Montuschi, P and Dahlen, S-E and Djukanovic, R and Sterk, P and Maitland-Van, Der Zee AH and Chotirmall, SH and Howarth, P and Adcock, IM and Chung, KF and U-BIOPRED, consortium},
doi = {10.1111/all.15776},
journal = {Allergy},
pages = {2906--2920},
title = {Haemophilus influenzae and Moraxella catarrhalis in sputum of severe asthma with inflammasome and neutrophil activation},
url = {http://dx.doi.org/10.1111/all.15776},
volume = {78},
year = {2023}
}

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TY  - JOUR
AB  - BACKGROUND: Because of altered airway microbiome in asthma, we analysed the bacterial species in sputum of patients with severe asthma. METHODS: Whole genome sequencing was performed on induced sputum from non-smoking (SAn) and current or ex-smoker (SAs/ex) severe asthma patients, mild/moderate asthma (MMA) and healthy controls (HC). Data were analysed by asthma severity, inflammatory status and transcriptome-associated clusters (TACs). RESULTS: α-diversity at the species level was lower in SAn and SAs/ex, with an increase in Haemophilus influenzae and Moraxella catarrhalis, and Haemophilus influenzae and Tropheryma whipplei, respectively, compared to HC. In neutrophilic asthma, there was greater abundance of Haemophilus influenzae and Moraxella catarrhalis and in eosinophilic asthma, Tropheryma whipplei was increased. There was a reduction in α-diversity in TAC1 and TAC2 that expressed high levels of Haemophilus influenzae and Tropheryma whipplei, and Haemophilus influenzae and Moraxella catarrhalis, respectively, compared to HC. Sputum neutrophils correlated positively with Moraxella catarrhalis and negatively with Prevotella, Neisseria and Veillonella species and Haemophilus parainfluenzae. Sputum eosinophils correlated positively with Tropheryma whipplei which correlated with pack-years of smoking. α- and β-diversities were stable at one year. CONCLUSIONS: Haemophilus influenzae and Moraxella catarrhalis were more abundant in severe neutrophilic asthma and TAC2 linked to inflammasome and neutrophil activation, while Haemophilus influenzae and Tropheryma whipplei were highest in SAs/ex and in TAC1 associated with highest expression of IL-13 type 2 and ILC2 signatures with the abundance of Tropheryma whipplei correlating positively with sputum eosinophils. Whether these bacterial species drive the inflammatory response in asthma needs evaluation.
AU  - Versi,A
AU  - Ivan,FX
AU  - Abdel-Aziz,MI
AU  - Bates,S
AU  - Riley,J
AU  - Baribaud,F
AU  - Kermani,NZ
AU  - Montuschi,P
AU  - Dahlen,S-E
AU  - Djukanovic,R
AU  - Sterk,P
AU  - Maitland-Van,Der Zee AH
AU  - Chotirmall,SH
AU  - Howarth,P
AU  - Adcock,IM
AU  - Chung,KF
AU  - U-BIOPRED,consortium
DO  - 10.1111/all.15776
EP  - 2920
PY  - 2023///
SN  - 0105-4538
SP  - 2906
TI  - Haemophilus influenzae and Moraxella catarrhalis in sputum of severe asthma with inflammasome and neutrophil activation
T2  - Allergy
UR  - http://dx.doi.org/10.1111/all.15776
UR  - https://www.ncbi.nlm.nih.gov/pubmed/37287344
UR  - http://hdl.handle.net/10044/1/104945
VL  - 78
ER  -

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