Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tanzer:2003:10.1007/s10142-003-0089-3,
author = {Tanzer, MM and Arst, HN and Skalchunes, AR and Coffin, M and Darveaux, BA and Heiniger, RW and Shuster, JR},
doi = {10.1007/s10142-003-0089-3},
journal = {Funct Integr Genomics},
pages = {160--170},
title = {Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi.},
url = {http://dx.doi.org/10.1007/s10142-003-0089-3},
volume = {3},
year = {2003}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We describe a method for gene function discovery and chemical mode-of-action analysis via nutrient utilization using a high throughput Nutritional Profiling platform suitable for filamentous microorganisms. We have optimized the growth conditions for each fungal species to produce reproducible optical density growth measurements in microtiter plates. We validated the Nutritional Profiling platform using a nitrogen source utilization assay to analyze 21 Aspergillus nidulans strains with mutations in the master nitrogen regulatory gene, areA. Analysis of these data accurately reproduced expected results and provided new data to demonstrate that this platform is suitable for fine level phenotyping of filamentous fungi. Next, we analyzed the differential responses of two fungal species to a glutamine synthetase inhibitor, illustrating chemical mode-of-action analysis. Finally, a comparative phenotypic study was performed to characterize carbon catabolite repression in four fungal species using a carbon source utilization assay. The results demonstrate differentiation between two Aspergillus species and two diverse plant pathogens and provide a wealth of new data on fungal nutrient utilization. Thus, these assays can be used for gene function and chemical mode-of-action analysis at the whole organism level as well as interspecies comparisons in a variety of filamentous fungi. Additionally, because uniform distribution of growth within wells is maintained, comparisons between yeast and filamentous forms of a single organism can be performed.
AU  - Tanzer,MM
AU  - Arst,HN
AU  - Skalchunes,AR
AU  - Coffin,M
AU  - Darveaux,BA
AU  - Heiniger,RW
AU  - Shuster,JR
DO  - 10.1007/s10142-003-0089-3
EP  - 170
PY  - 2003///
SN  - 1438-793X
SP  - 160
TI  - Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi.
T2  - Funct Integr Genomics
UR  - http://dx.doi.org/10.1007/s10142-003-0089-3
UR  - https://www.ncbi.nlm.nih.gov/pubmed/12898394
VL  - 3
ER  -
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