Imperial College London
Alternate

DrJonathanBaker

Faculty of Medicine, National Heart & Lung Institute

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 7790jonathan.baker

 
 
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Location

 

223Guy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ho:2023:10.1038/s42003-023-04845-8,
author = {Ho, V and Baker, J and Willison, K and Barnes, P and Donnelly, L and Klug, D},
doi = {10.1038/s42003-023-04845-8},
journal = {Communications Biology},
pages = {1--11},
title = {Single cell quantification of microRNA from small numbers of non-invasively sampled primary human cells},
url = {http://dx.doi.org/10.1038/s42003-023-04845-8},
volume = {6},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Expression levels of microRNAs (miRNAs) in single cells are low and conventional miRNA detection methods require amplification that can be complex, time-consuming, costly and may bias results. Single cell microfluidic platforms have been developed; however, current approaches are unable to absolutely quantify single miRNA molecules expressed in single cells. Herein, we present an amplification-free sandwich hybridisation assay to detect single miRNA molecules in single cells using a microfluidic platform that optically traps and lyses individual cells. Absolute quantification of miR-21 and miR-34a molecules was achieved at a single cell level in human cell lines and validated using real-time qPCR. The sensitivity of the assay was demonstrated by quantifying single miRNA molecules in nasal epithelial cells and CD3+ T-cells, as well as nasal fluid collected non-invasively from healthy individuals. This platform requires ~50 cells or ~30 µL biofluid and can be extended for other miRNA targets therefore it could monitor miRNA levels in disease progression or clinical studies.
AU  - Ho,V
AU  - Baker,J
AU  - Willison,K
AU  - Barnes,P
AU  - Donnelly,L
AU  - Klug,D
DO  - 10.1038/s42003-023-04845-8
EP  - 11
PY  - 2023///
SN  - 2399-3642
SP  - 1
TI  - Single cell quantification of microRNA from small numbers of non-invasively sampled primary human cells
T2  - Communications Biology
UR  - http://dx.doi.org/10.1038/s42003-023-04845-8
UR  - https://www.nature.com/articles/s42003-023-04845-8
UR  - http://hdl.handle.net/10044/1/104073
VL  - 6
ER  -
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