Imperial College London
Alternate

Dr Kambiz N. Alavian, PhD, SFHEA, FLS, FRSB

Faculty of Medicine, Department of Brain Sciences

Reader in Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 7006k.alavian Website

 
 
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Assistant

 

Mrs Hadeel Abdeen +44 (0)20 7594 7014

 
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Location

 

E507Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Alavian:2011:10.1038/ncb2330,
author = {Alavian, KN and Li, H and Collis, L and Bonanni, L and Zeng, L and Sacchetti, S and Lazrove, E and Nabili, P and Flaherty, B and Graham, M and Chen, Y and Messerli, SM and Mariggio, MA and Rahner, C and McNay, E and Shore, GC and Smith, PJS and Hardwick, JM and Jonas, EA},
doi = {10.1038/ncb2330},
journal = {Nature Cell Biology},
pages = {1224--1233},
title = {Bcl-x(L) regulates metabolic efficiency of neurons through interaction with the mitochondrial F1F0 ATP synthase},
url = {http://dx.doi.org/10.1038/ncb2330},
volume = {13},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Anti-apoptotic Bcl2 family proteins such as Bcl-xL protect cells from death by sequestering apoptotic molecules, but also contribute to normal neuronal function. We find in hippocampal neurons that Bcl-xL enhances the efficiency of energy metabolism. Our evidence indicates that Bcl-xLinteracts directly with the β-subunit of the F1FO ATP synthase, decreasing an ion leak within the F1FO ATPase complex and thereby increasing net transport of H+ by F1FO during F1FO ATPase activity. By patch clamping submitochondrial vesicles enriched in F1FO ATP synthase complexes, we find that, in the presence of ATP, pharmacological or genetic inhibition of Bcl-xL activity increases the membrane leak conductance. In addition, recombinant Bcl-xL protein directly increases the level of ATPase activity of purified synthase complexes, and inhibition of endogenous Bcl-xL decreases the level of F1FO enzymatic activity. Our findings indicate that increased mitochondrial efficiency contributes to the enhanced synaptic efficacy found in Bcl-xL-expressing neurons.
AU  - Alavian,KN
AU  - Li,H
AU  - Collis,L
AU  - Bonanni,L
AU  - Zeng,L
AU  - Sacchetti,S
AU  - Lazrove,E
AU  - Nabili,P
AU  - Flaherty,B
AU  - Graham,M
AU  - Chen,Y
AU  - Messerli,SM
AU  - Mariggio,MA
AU  - Rahner,C
AU  - McNay,E
AU  - Shore,GC
AU  - Smith,PJS
AU  - Hardwick,JM
AU  - Jonas,EA
DO  - 10.1038/ncb2330
EP  - 1233
PY  - 2011///
SN  - 1465-7392
SP  - 1224
TI  - Bcl-x(L) regulates metabolic efficiency of neurons through interaction with the mitochondrial F1F0 ATP synthase
T2  - Nature Cell Biology
UR  - http://dx.doi.org/10.1038/ncb2330
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000295617900012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/ncb2330
UR  - http://hdl.handle.net/10044/1/103354
VL  - 13
ER  -
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