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@article{Broto:2022:10.1038/s41467-022-29574-0,
author = {Broto, A and Gaspari, E and Miravet-Verde, S and Martins, dos Santos VAP and Isalan, M},
doi = {10.1038/s41467-022-29574-0},
journal = {Nature Communications},
title = {A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications},
url = {http://dx.doi.org/10.1038/s41467-022-29574-0},
volume = {13},
year = {2022}
}

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TY  - JOUR
AB  - Mycoplasmas have exceptionally streamlined genomes and are strongly adapted to their many hosts, which provide them with essential nutrients. Owing to their relative genomic simplicity, Mycoplasmas have been used to develop chassis for biotechnological applications. However, the dearth of robust and precise toolkits for genomic manipulation and tight regulation has hindered any substantial advance. Herein we describe the construction of a robust genetic toolkit for M. pneumoniae, and its successful deployment to engineer synthetic gene switches that control and limit Mycoplasma growth, for biosafety containment applications. We found these synthetic gene circuits to be stable and robust in the long-term, in the context of a minimal cell. With this work, we lay a foundation to develop viable and robust biosafety systems to exploit a synthetic Mycoplasma chassis for live attenuated vectors for therapeutic applications.
AU  - Broto,A
AU  - Gaspari,E
AU  - Miravet-Verde,S
AU  - Martins,dos Santos VAP
AU  - Isalan,M
DO  - 10.1038/s41467-022-29574-0
PY  - 2022///
SN  - 2041-1723
TI  - A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-022-29574-0
UR  - http://hdl.handle.net/10044/1/96675
VL  - 13
ER  -

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Professor Mark Isalan - Deputy Head of Department

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