Imperial College London

Michael J Jeger

Faculty of Natural SciencesDepartment of Life Sciences (Silwood Park)

Emeritus Professor of Horticulture
 
 
 
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Contact

 

+44 (0)1398 332 941m.jeger Website

 
 
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Location

 

Home working 13 Brook Street, Bampton, Devon EX16 9LUSilwood ParkSilwood Park

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Summary

 

Publications

Citation

BibTex format

@inbook{Allen:2012,
author = {Allen, EJ and Howell, R and Fernández-Fernández, F and Jeger, MJ},
booktitle = {Apples: Nutrition, Consumption and Health},
pages = {141--158},
title = {The use of microsatellite markers and morphological traits in characterising and maintaining genetic diversity in a cultivated apple germplasm collection},
year = {2012}
}

RIS format (EndNote, RefMan)

TY  - CHAP
AB - The consumption of apple has demonstrated benefits for human nutrition and health which make the characterisation and maintenance of genetic diversity of the utmost importance. The aim of this study was to investigate whether multivariate analysis of characterisation data obtained using microsatellite markers (SSR) provided useful information for the management of a field gene-bank collection of apple varieties; and as a subsidiary aim, the effectiveness of this approach was compared with a similar analysis of morphological traits. SSR characterization data at 12 loci collected from 85 accessions in the UK National Fruit Collections were analysed along with morphological character scores that were available for a subset of 28 of those accessions. In addition, the application of SSR polymorphisms for resolving queries that cannot be answered on the basis of morphological data alone, e.g. identification of duplicates and relationships between accessions of unknown origin, was investigated. For the SSR loci tested there was generally a high level of heterozygosity, but at some loci alleles were not associating randomly. Sets of diploid varieties that were clonally derived were identified. Fifteen of the varieties were revealed to be triploids, some not previously known. Multivariate analysis using Principal Co-ordinate Analysis presented a reasonable model for the genetic data, but did not reveal groupings of the apple accessions. Similarly there were no discernible groups formed with the morphological data and the positioning of accessions differed from those formed with the genetic data. The uses of such analyses in management of genetic diversity is discussed in relation to historical literature, parentage analysis and the use of collection by plant breeders in improving nutritional quality and other traits in cultivated apple. © 2012 Nova Science Publishers, Inc. All rights reserved.
AU - Allen,EJ
AU - Howell,R
AU - Fernández-Fernández,F
AU - Jeger,MJ
EP - 158
PY - 2012///
SN - 9781619427099
SP - 141
TI - The use of microsatellite markers and morphological traits in characterising and maintaining genetic diversity in a cultivated apple germplasm collection
T1 - Apples: Nutrition, Consumption and Health
ER -