Publications
179 results found
Sharma J, Panico M, Barber J, et al., 1997, Purification and determination of intact molecular mass by electrospray ionization mass spectrometry of the photosystem II reaction center subunits, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 33153-33157, ISSN: 0021-9258
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- Citations: 37
Sharma J, Panico M, Shipton CA, et al., 1997, Primary structure characterization of the photosystem II D1 and D2 subunits, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 33158-33166, ISSN: 0021-9258
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- Citations: 69
Morris HR, Etienne AT, Panico M, et al., 1997, Hypothalamic hypertensive factor - An inhibitor of nitric oxide synthase activity, HYPERTENSION, Vol: 30, Pages: 1493-1498, ISSN: 0194-911X
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- Citations: 3
Morris HR, Paxton T, Panico M, et al., 1997, A novel geometry mass spectrometer, the Q-TOF, for low-femtomole/attomole-range biopolymer sequencing, JOURNAL OF PROTEIN CHEMISTRY, Vol: 16, Pages: 469-479, ISSN: 0277-8033
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- Citations: 80
Sharma J, Panico M, Barber J, et al., 1997, Characterization of the low molecular weight photosystem II reaction center subunits and their light-induced modifications by mass spectrometry, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 3935-3943, ISSN: 0021-9258
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- Citations: 52
Morris HR, Dell A, Easton RL, et al., 1996, Gender-specific glycosylation of human glycodelin affects its contraceptive activity, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 271, Pages: 32159-32167, ISSN: 0021-9258
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- Citations: 135
Stein T, Vater J, Kruft V, et al., 1996, The multiple carrier model of nonribosomal peptide biosynthesis at modular multienzymatic templates, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 271, Pages: 15428-15435, ISSN: 0021-9258
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- Citations: 166
Matthews JR, Botting CH, Panico M, et al., 1996, Inhibition of NF-kappa B DNA binding by nitric oxide, NUCLEIC ACIDS RESEARCH, Vol: 24, Pages: 2236-2242, ISSN: 0305-1048
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- Citations: 430
Stimson E, Virji M, Barker S, et al., 1996, Discovery of a novel protein modification: alpha-glycerophosphate is a substituent of meningococcal pilin, BIOCHEMICAL JOURNAL, Vol: 316, Pages: 29-33, ISSN: 0264-6021
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- Citations: 71
Burrows L, Knight R, Polokoff E, et al., 1996, Expanding the donor pool with the use of en bloc pediatric kidneys in adult recipients, TRANSPLANTATION PROCEEDINGS, Vol: 28, Pages: 173-174, ISSN: 0041-1345
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- Citations: 19
Ruoppolo M, Torella C, Kanda F, et al., 1996, Identification of disulphide bonds in the refolding of bovine pancreatic RNase A, FOLDING & DESIGN, Vol: 1, Pages: 381-390, ISSN: 1359-0278
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- Citations: 25
DELL A, MORRIS HR, EASTON RL, et al., 1995, STRUCTURAL-ANALYSIS OF THE OLIGOSACCHARIDES DERIVED FROM GLYCODELIN, A HUMAN GLYCOPROTEIN WITH POTENT IMMUNOSUPPRESSIVE AND CONTRACEPTIVE ACTIVITIES, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 270, Pages: 24116-24126, ISSN: 0021-9258
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- Citations: 237
STIMSON E, VIRJI M, MAKEPEACE K, et al., 1995, MENINGOCOCCAL PILIN - A GLYCOPROTEIN SUBSTITUTED WITH DIGALACTOSYL 2,4-DIACETAMIDO-2,4,6-TRIDEOXYHEXOSE, MOLECULAR MICROBIOLOGY, Vol: 17, Pages: 1201-1214, ISSN: 0950-382X
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- Citations: 221
LINDO VS, KAKKAR VV, LEARMONTH M, et al., 1995, ANTITHROMBIN-TRI (ALA382 TO THR) CAUSING SEVERE THROMBOEMBOLIC TENDENCY UNDERGOES THE S-TO-R TRANSITION AND IS ASSOCIATED WITH A PLASMA-INACTIVE HIGH-MOLECULAR-WEIGHT COMPLEX OF AGGREGATED ANTITHROMBIN, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 89, Pages: 589-601, ISSN: 0007-1048
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- Citations: 26
BURROWS L, KNIGHT R, KUPFER S, et al., 1995, THE DIAGNOSTIC-ACCURACY OF URINE CYTOLOGICAL ANALYSIS IN THE RAPID ASSESSMENT OF ACUTE RENAL-ALLOGRAFT DYSFUNCTION, TRANSPLANTATION PROCEEDINGS, Vol: 27, Pages: 1044-1045, ISSN: 0041-1345
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- Citations: 3
BOBBIONIHARSCH E, FRUTIGER S, HUGHES G, et al., 1995, PHYSIOLOGICAL CONCENTRATIONS OF OXYTOCIN POWERFULLY STIMULATE INSULIN-SECRETION IN-VITRO, ENDOCRINE, Vol: 3, Pages: 55-59, ISSN: 0969-711X
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- Citations: 8
CLARK GF, PATANKAR M, OEHNINGER S, et al., 1995, THE SELECTIN-LIKE CARBOHYDRATE-BINDING SPECIFICITY OF INITIAL HUMAN SPERM ZONA-PELLUCIDA BINDING AND ITS IMPLICATIONS, BIOLOGY OF REPRODUCTION, Vol: 52, Pages: 120-120, ISSN: 0006-3363
VARGAS HM, BREZENOFF HE, MORRIS HR, et al., 1994, EFFECT OF HEMICHOLINIUM-3 ON THE HYPOTHALAMIC CONCENTRATION OF A CYTOCHEMICALLY DETECTABLE GLUCOSE-6-PHOSPHATE DEHYDROGENASE-STIMULATING SUBSTANCE, JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, Vol: 24, Pages: 773-778, ISSN: 0160-2446
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- Citations: 2
STEIN T, VATER J, KRUFT V, et al., 1994, DETECTION OF 4'-PHOSPHOPANTETHEINE AT THE THIOESTER BINDING-SITE FOR L-VALINE OF GRAMICIDINS SYNTHETASE-2, FEBS LETTERS, Vol: 340, Pages: 39-44, ISSN: 0014-5793
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- Citations: 61
DELL A, PANICO M, MCDOWELL RA, et al., 1994, APPLICATIONS OF MASS-SPECTROMETRY TO COMPLEX CARBOHYDRATES, Alfred Benzon Symposium on Complex Carbohydrates in Drug Research, Publisher: MUNKSGAARD, Pages: 45-59, ISSN: 0105-3639
DELL A, REASON AJ, KHOO KH, et al., 1994, MASS-SPECTROMETRY OF CARBOHYDRATE-CONTAINING BIOPOLYMERS, GUIDE TO TECHNIQUES IN GLYCOBIOLOGY, Vol: 230, Pages: 108-132, ISSN: 0076-6879
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- Citations: 233
DELL A, EASTON R, KHOO KH, et al., 1993, APPLICATIONS OF MASS-SPECTROMETRY TO GLYCOBIOLOGY, GLYCOCONJUGATE JOURNAL, Vol: 10, Pages: 275-276, ISSN: 0282-0080
Siddiqui KS, Rangarajan M, Hartley BS, et al., 1993, Arthrobacter D-xylose isomerase: partial proteolysis with thermolysin., Biochem J, Vol: 289 ( Pt 1), Pages: 201-208, ISSN: 0264-6021
The pattern and kinetics of partial proteolysis of Arthrobacter D-xylose isomerase tetramer was studied in order to determine the flexibility of surface loops that may control its stability. It was completely resistant to trypsin, chymotrypsin and elastase at 37 degrees C, but thermolysin cleaved specifically and quantitatively at Thr-347-Leu-348 between helices 10 and 11 to remove 47 residues from the C-terminus of each 43.3 kDa subunit. At high temperatures, helices 9 and 10 were removed from each 38 kDa subunit to give a 36 kDa tetramer. The kinetics of nicking by thermolysin indicated that the Thr-347-Leu-348 loop is locked at low temperatures, but 'melts' at 25 degrees C and is fully flexible above 34 degrees C. The flexibility appears to be associated with binding of Ca2+ ions at the active site, since Co2+, Mg2+ and xylitol protect in proportion to their ability to displace Ca2+. The missing C-terminal helices make many intersubunit contacts that appear in the structure to stabilize the tetramer, but the properties of the purified nicked proteins are almost indistinguishable from the native enzyme. Both the 38 kDa tetramer and the 36 kDa tetramer are identically active and dissociate similarly in urea or SDS to fully active dimers, but the nicked dimers are slightly less stable to urea at 62 degrees C. In the Mg2+ form the thermostability of the 38 kDa tetramer is identical with that of the native enzyme, but the 36 kDa tetramer has a slightly lower 'melting point' (70 degrees C versus 80 degrees C), which may be due to unravelling from the end of helix 8. Since elimination of all the C-terminal helices and many intersubunit contacts has so little effect, one can conclude that the 'weak point' that controls the protein's thermostability lies within the N-terminal beta-barrel domain.
SIDDIQUI KS, RANGARAJAN M, HARTLEY BS, et al., 1993, ARTHROBACTER D-XYLOSE ISOMERASE - PARTIAL PROTEOLYSIS WITH THERMOLYSIN, BIOCHEMICAL JOURNAL, Vol: 289, Pages: 201-208, ISSN: 0264-6021
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- Citations: 17
de Wardener HE, Holland S, Alaghband-Zadeh J, et al., 1993, A possible connection between an increased concentration of a cytochemically detectable substance in the hypothalamus of the spontaneously hypertensive rat and certain cerebral cholinergic disturbances., J Cardiovasc Pharmacol, Vol: 22 Suppl 2, Pages: S109-S111, ISSN: 0160-2446
The hypothalamus of the spontaneously hypertensive rat (SHR) contains an increased concentration of a cytochemically detectable choline-like substance, the physicochemical properties of which are similar to the choline analogue dimethyl methylene immonium. In the hypothalamus of the adult SHR there is an increased turnover of acetylcholine in the cholinergic pressor posterior hypothalamic nucleus and the neurons of the dorsomedial cholinergic depressor nucleus are atrophic, possibly due to diminished cholinergic activity. Both of these changes are pressor. It is suggested that an increased hypothalamic content of a choline-like analogue might either be responsible for, or an indicator of, these abnormalities.
DEWARDENER HE, HOLLAND S, ALAGHBANDZADEH J, et al., 1993, A POSSIBLE CONNECTION BETWEEN AN INCREASED CONCENTRATION OF A CYTOCHEMICALLY DETECTABLE SUBSTANCE IN THE HYPOTHALAMUS OF THE SPONTANEOUSLY HYPERTENSIVE RAT AND CERTAIN CEREBRAL CHOLINERGIC DISTURBANCES, SATELLITE MEETING ON NATRIURETIC FACTORS AND HYPERTENSION, AT THE 14TH SCIENTIFIC MEETING OF THE INTERNATIONAL-SOC-OF-HYPERTENSION, Publisher: LIPPINCOTT-RAVEN PUBL, Pages: S109-S111, ISSN: 0160-2446
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- Citations: 2
REASON AJ, MORRIS HR, PANICO M, et al., 1992, LOCALIZATION OF O-GLCNAC MODIFICATION ON THE SERUM RESPONSE TRANSCRIPTION FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 267, Pages: 16911-16921, ISSN: 0021-9258
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- Citations: 119
OLDS RJ, LANE DA, CASO R, et al., 1992, ANTITHROMBIN-III BUDAPEST - A SINGLE AMINO-ACID SUBSTITUTION (429PRO TO LEU) IN A REGION HIGHLY CONSERVED IN THE SERPIN FAMILY, BLOOD, Vol: 79, Pages: 1206-1212, ISSN: 0006-4971
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- Citations: 51
ROQUEMORE EP, DELL A, MORRIS HR, et al., 1992, VERTEBRATE LENS ALPHA-CRYSTALLINS ARE MODIFIED BY O-LINKED N-ACETYLGLUCOSAMINE, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 267, Pages: 555-563, ISSN: 0021-9258
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- Citations: 98
Reason AJ, Blench IP, Haltiwanger RS, et al., 1991, High-sensitivity FAB-MS strategies for O-GlcNAc characterization., Glycobiology, Vol: 1, Pages: 585-594, ISSN: 0959-6658
In this paper we report the first application of fast atom bombardment mass spectrometry (FAB-MS) to O-linked N-acetylglucosamine (O-GlcNAc)-bearing glycopeptides. Using N-acetylgalactosamine (GalNAc)- and Gal-GalNAc-containing glycopeptides (isolated from Tn glycophorin and desialylated normal glycophorin, respectively) as readily available model compounds, rapid and sensitive derivatization/FAB-MS strategies applicable to serine/threonine-rich glycopeptides have been devised. Peptides and glycopeptides were propionylated in a 1 min reaction using a mixture of trifluoroacetic anhydride and propionic acid, and the product mixtures were analysed directly by FAB-MS. Glycopeptides and peptides rich in hydroxylated residues afforded characteristic clusters of molecular ions at high sensitivity. Additional sensitivity enhancement was achieved by prior esterification of carboxyl groups. These methods were used in a study of O-GlcNAc glycopeptides produced by purified O-GlcNAc transferase addition of GlcNAc to the synthetic peptides YSDSPSTST and YSGSPSTST in which Y is tyrosine, S is serine, D is aspartic acid, P is proline, T is threonine and G is glycine. The propionyl derivatives afforded high-quality spectra which unequivocally showed that the majority of the glycopeptides were substituted with a single GlcNAc residue. Low pmol quantities of material gave detectable signals. The propionylation/FAB-MS procedure has been combined with gas-phase sequencing strategies and shows promise for defining the sites of glycosylation of O-GlcNAc glycopeptides that are available in limited quantities.
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