Imperial College London
Portrait Picture

Professor Michael A. ("Mike") Skinner

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor in Virology
 
 
 
//

Contact

 

+44 (0)20 7594 3938m.skinner Website

 
 
//

Assistant

 

Mrs Yasmin Mallu +44 (0)20 7594 3972

 
//

Location

 

315Medical SchoolSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Dulwich:2017:10.1099/jgv.0.000979,
author = {Dulwich, KL and Giotis, ES and Gray, A and Nair, V and Skinner, MA and Broadbent, AJ},
doi = {10.1099/jgv.0.000979},
journal = {Journal of General Virology},
pages = {2918--2930},
title = {Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV).},
url = {http://dx.doi.org/10.1099/jgv.0.000979},
volume = {98},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Infectious bursal disease virus (IBDV) belongs to the family Birnaviridae and is economically important to the poultry industry worldwide. IBDV infects B cells in the bursa of Fabricius (BF), causing immunosuppression and morbidity in young chickens. In addition to strains that cause classical Gumboro disease, the so-called 'very virulent' (vv) strain, also in circulation, causes more severe disease and increased mortality. IBDV has traditionally been controlled through the use of live attenuated vaccines, with attenuation resulting from serial passage in non-lymphoid cells. However, the factors that contribute to the vv or attenuated phenotypes are poorly understood. In order to address this, we aimed to investigate host cell-IBDV interactions using a recently described chicken primary B-cell model, where chicken B cells are harvested from the BF and cultured ex vivo in the presence of chicken CD40L. We demonstrated that these cells could support the replication of IBDV when infected ex vivo in the laboratory. Furthermore, we evaluated the gene expression profiles of B cells infected with an attenuated strain (D78) and a very virulent strain (UK661) by microarray. We found that key genes involved in B-cell activation and signalling (TNFSF13B, CD72 and GRAP) were down-regulated following infection relative to mock, which we speculate could contribute to IBDV-mediated immunosuppression. Moreover, cells responded to infection by expressing antiviral type I IFNs and IFN-stimulated genes, but the induction was far less pronounced upon infection with UK661, which we speculate could contribute to its virulence.
AU  - Dulwich,KL
AU  - Giotis,ES
AU  - Gray,A
AU  - Nair,V
AU  - Skinner,MA
AU  - Broadbent,AJ
DO  - 10.1099/jgv.0.000979
EP  - 2930
PY  - 2017///
SN  - 1465-2099
SP  - 2918
TI  - Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV).
T2  - Journal of General Virology
UR  - http://dx.doi.org/10.1099/jgv.0.000979
UR  - http://hdl.handle.net/10044/1/54691
VL  - 98
ER  -
Download