Imperial College London
Alternate

Professor Molly Stevens

Faculty of Engineering, Department of Materials

Professor of Biomedical Materials and Regenerative Medicine
 
 
 
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Contact

 

+44 (0)20 7594 6804m.stevens

 
 
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Location

 

208Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Broto:2022:10.1038/s41565-022-01179-0,
author = {Broto, M and Kaminski, MM and Adrianus, C and Kim, N and Greensmith, R and Dissanayake-Perera, S and Schubert, AJ and Tan, X and Kim, H and Dighe, AS and Collins, JJ and Stevens, M},
doi = {10.1038/s41565-022-01179-0},
journal = {Nature Nanotechnology},
pages = {1120--1126},
title = {Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs},
url = {http://dx.doi.org/10.1038/s41565-022-01179-0},
volume = {17},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers associated withhuman diseases. This is achieved through the binding of guide RNAs to a complementarysequence which activates Cas enzymes to cleave reporter molecules. Currently, most CRISPRbased diagnostics rely on target preamplification to reach sufficient sensitivity for clinicalapplications. This limits quantification capability and adds complexity to the reaction chemistry.Here, we show the combination of a CRISPR/Cas-based reaction with a Nanozyme-LinkedImmunoSorbent Assay which allows for the quantitative and colorimetric readout of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature(CrisprZyme). We demonstrate CrisprZyme is easily adaptable to a lateral-flow-based readoutand different Cas enzymes, and enables the sensing of non-coding RNAs including microRNAs,long non-coding RNAs and circular RNAs. We utilise this platform to identify patients with acutemyocardial infarction and to monitor cellular differentiation in vitro and in tissue biopsies fromprostate cancer patients. We anticipate that CrisprZyme has significant potential as a universallyapplicable signal catalyst for CRISPR-based diagnostics which will expand the spectrum oftargets for preamplification-free, quantitative detection.
AU  - Broto,M
AU  - Kaminski,MM
AU  - Adrianus,C
AU  - Kim,N
AU  - Greensmith,R
AU  - Dissanayake-Perera,S
AU  - Schubert,AJ
AU  - Tan,X
AU  - Kim,H
AU  - Dighe,AS
AU  - Collins,JJ
AU  - Stevens,M
DO  - 10.1038/s41565-022-01179-0
EP  - 1126
PY  - 2022///
SN  - 1748-3387
SP  - 1120
TI  - Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs
T2  - Nature Nanotechnology
UR  - http://dx.doi.org/10.1038/s41565-022-01179-0
UR  - http://hdl.handle.net/10044/1/97651
VL  - 17
ER  -
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