Imperial College London
Alternate

ProfessorMarkJohnson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Chair in Obstetrics
 
 
 
//

Contact

 

+44 (0)20 3315 7887mark.johnson

 
 
//

Location

 

H3.35Chelsea and Westminster HospitalChelsea and Westminster Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Waldorf:2015:10.1016/j.ajog.2015.08.028,
author = {Waldorf, KMA and Singh, N and Mohan, AR and Young, RC and Ngo, L and Das, A and Tsai, J and Bansal, A and Paolella, L and Herbert, BR and Sooranna, SR and Gough, M and Astley, C and Vogel, K and Baldessari, AE and Bammler, TK and MacDonald, J and Gravett, MG and Rajagopal, L and Johnson, MR},
doi = {10.1016/j.ajog.2015.08.028},
journal = {American Journal of Obstetrics and Gynecology},
pages = {830.e1--830.e19},
title = {Uterine overdistention induces preterm labor mediated by inflammation: observations in pregnant women and nonhuman primates},
url = {http://dx.doi.org/10.1016/j.ajog.2015.08.028},
volume = {213},
year = {2015}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ObjectiveUterine overdistention is thought to induce preterm labor in women with twin and multiple pregnancies, but the pathophysiology remains unclear. We investigated for the first time the pathogenesis of preterm birth associated with rapid uterine distention in a pregnant nonhuman primate model.Study DesignA nonhuman primate model of uterine overdistention was created using preterm chronically catheterized pregnant pigtail macaques (Macaca nemestrina) by inflation of intraamniotic balloons (N = 6), which were compared to saline controls (N = 5). Cesarean delivery was performed due to preterm labor or at experimental end. Microarray, quantitative reverse transcriptase polymerase chain reaction, Luminex (Austin, TX), and enzyme-linked immunosorbent assay were used to measure messenger RNA (mRNA) and/or protein levels from monkey (amniotic fluid, myometrium, maternal plasma) and human (amniocytes, amnion, myometrium) tissues. Statistical analysis employed analysis of covariance and Wilcoxon rank sum. Biomechanical forces were calculated using the law of Laplace.ResultsPreterm labor occurred in 3 of 6 animals after balloon inflation and correlated with greater balloon volume and uterine wall stress. Significant elevations of inflammatory cytokines and prostaglandins occurred following uterine overdistention in an “inflammatory pulse” that correlated with preterm labor (interleukin [IL]-1β, tumor necrosis factor [TNF]-α, IL-6, IL-8, CCL2, prostaglandin E2, prostaglandin F2α, all P < .05). A similar inflammatory response was observed in amniocytes in vitro following mechanical stretch (IL1β, IL6, and IL8 mRNA multiple time points, P < .05), in amnion of women with polyhydramnios (IL6 and TNF mRNA, P < .05) and in amnion (TNF-α) and myometrium of women with twins in early labor (IL6, IL8, CCL2, all P < .05). Genes differentially expressed in the nonhuman primate after balloon inflation and in women with polyhydramnios
AU  - Waldorf,KMA
AU  - Singh,N
AU  - Mohan,AR
AU  - Young,RC
AU  - Ngo,L
AU  - Das,A
AU  - Tsai,J
AU  - Bansal,A
AU  - Paolella,L
AU  - Herbert,BR
AU  - Sooranna,SR
AU  - Gough,M
AU  - Astley,C
AU  - Vogel,K
AU  - Baldessari,AE
AU  - Bammler,TK
AU  - MacDonald,J
AU  - Gravett,MG
AU  - Rajagopal,L
AU  - Johnson,MR
DO  - 10.1016/j.ajog.2015.08.028
EP  - 1
PY  - 2015///
SN  - 1097-6868
SP  - 830
TI  - Uterine overdistention induces preterm labor mediated by inflammation: observations in pregnant women and nonhuman primates
T2  - American Journal of Obstetrics and Gynecology
UR  - http://dx.doi.org/10.1016/j.ajog.2015.08.028
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000365764900013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0002937815008947?via%3Dihub
UR  - http://hdl.handle.net/10044/1/39112
VL  - 213
ER  -
Download