Imperial College London
Alternate

Michael A Crawford PhD, FRSB, FRCPath

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)7725 250 541michael.crawford Website CV

 
 
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Location

 

H 3.34Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moodley:2009:10.1177/026010600902000206,
author = {Moodley, T and Vella, C and Djahanbakhch, O and Branford-White, CJ and Crawford, MA},
doi = {10.1177/026010600902000206},
journal = {Nutr Health},
pages = {167--185},
title = {Arachidonic and docosahexaenoic acid deficits in preterm neonatal mononuclear cell membranes. Implications for the immune response at birth.},
url = {http://dx.doi.org/10.1177/026010600902000206},
volume = {20},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Preterm neonates are more susceptible to infection than term neonates. Arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) are biologically active components of cell membrane phospholipids. Arachidonic acid is a substrate for the synthesis of eicosanoids, potent regulators of immune function. Preterm babies may have a deficiency of arachidonic acid and docosahexaenoic acid, but the impact of this deficit on maturation of the immune system is unknown. To address this we explored links between placental provision of fatty acids to cord blood mononuclear cell (CBMC) membranes using gas chromatography (GC), and maturation of the immune response with gestational age by analysing lymphocyte subsets by flow cytometry. This is the first study to examine the lipid profile of the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) fractions of CBMC membranes from preterm neonates. The long chain polyunsaturated fatty acid (LCPUFA) composition of CBMC membranes was dominated by arachidonic acid in both PE (34%) and PC (15%) fractions in healthy term neonates (> or =37 weeks, n=9), whilst in healthy preterm neonates (<37 weeks, n=10) the level of arachidonic acid was significantly lower at 28.8% and 12.5% respectively (p<0.05). Preterm neonates (<37 weeks, n=23) also had significantly lower absolute numbers of CD4+ (p<0.05) leukocytes and CD4+ (p<0.01) and CD8+ (p<0.05) naïve T-cells than term (> or =37 weeks, n=24) neonates that correlated with gestational age (p<0.01-0.05).
AU  - Moodley,T
AU  - Vella,C
AU  - Djahanbakhch,O
AU  - Branford-White,CJ
AU  - Crawford,MA
DO  - 10.1177/026010600902000206
EP  - 185
PY  - 2009///
SN  - 0260-1060
SP  - 167
TI  - Arachidonic and docosahexaenoic acid deficits in preterm neonatal mononuclear cell membranes. Implications for the immune response at birth.
T2  - Nutr Health
UR  - http://dx.doi.org/10.1177/026010600902000206
UR  - https://www.ncbi.nlm.nih.gov/pubmed/19835110
VL  - 20
ER  -
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