Imperial College London
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Professor Peter Cherepanov

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Virology
 
 
 
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Contact

 

p.cherepanov

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Maskell:2015:10.1038/nature14495,
author = {Maskell, DP and Renault, L and Serrao, E and Lesbats, P and Matadeen, R and Hare, S and Lindemann, D and Engelman, AN and Costa, A and Cherepanov, P},
doi = {10.1038/nature14495},
journal = {Nature},
pages = {366--369},
title = {Structural basis for retroviral integration into nucleosomes},
url = {http://dx.doi.org/10.1038/nature14495},
volume = {523},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome1, 2. How the intasome interfaces with chromosomal DNA, which exists in the form of nucleosomal arrays, is currently unknown. Here we show that the prototype foamy virus (PFV) intasome is proficient at stable capture of nucleosomes as targets for integration. Single-particle cryo-electron microscopy reveals a multivalent intasome–nucleosome interface involving both gyres of nucleosomal DNA and one H2A–H2B heterodimer. While the histone octamer remains intact, the DNA is lifted from the surface of the H2A–H2B heterodimer to allow integration at strongly preferred superhelix location ±3.5 positions. Amino acid substitutions disrupting these contacts impinge on the ability of the intasome to engage nucleosomes in vitro and redistribute viral integration sites on the genomic scale. Our findings elucidate the molecular basis for nucleosome capture by the viral DNA recombination machinery and the underlying nucleosome plasticity that allows integration.
AU  - Maskell,DP
AU  - Renault,L
AU  - Serrao,E
AU  - Lesbats,P
AU  - Matadeen,R
AU  - Hare,S
AU  - Lindemann,D
AU  - Engelman,AN
AU  - Costa,A
AU  - Cherepanov,P
DO  - 10.1038/nature14495
EP  - 369
PY  - 2015///
SN  - 0028-0836
SP  - 366
TI  - Structural basis for retroviral integration into nucleosomes
T2  - Nature
UR  - http://dx.doi.org/10.1038/nature14495
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000357950900046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/nature14495
UR  - http://hdl.handle.net/10044/1/43749
VL  - 523
ER  -
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