Imperial College London
Alternate

Professor Sir Peter Barnes, FRS, FMedSci

Faculty of Medicine, National Heart & Lung Institute

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 7959p.j.barnes Website CV

 
 
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Assistant

 

Miss Carolyn Green +44 (0)20 7594 7959

 
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Location

 

227CGuy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2017:10.1136/thoraxjnl-2017-210741,
author = {Wang, Z and Singh, R and Miller, BE and Tal-Singer, R and Van, Horn S and Tomsho, L and Mackay, A and Allinson, JP and Webb, AJ and Brookes, AJ and George, LM and Barker, B and Kolsum, U and Donnelly, LE and Belchamber, K and Barnes, PJ and Singh, D and Brightling, CE and Donaldson, GC and Wedzicha, JA and Brown, JR and COPDMAP},
doi = {10.1136/thoraxjnl-2017-210741},
journal = {Thorax},
pages = {331--338},
title = {Sputum microbiome temporal variability and dysbiosis in chronic obstructive pulmonary disease exacerbations: an analysis of the COPDMAP study},
url = {http://dx.doi.org/10.1136/thoraxjnl-2017-210741},
volume = {73},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Recent studies suggest that lung microbiome dysbiosis, the disease associated disruption of the lung microbial community, might play a key role in chronic obstructive pulmonary disease (COPD) exacerbations. However, characterising temporal variability of the microbiome from large longitudinal COPD cohorts is needed to better understand this phenomenon. METHODS: We performed a 16S ribosomal RNA survey of microbiome on 716 sputum samples collected longitudinally at baseline and exacerbations from 281 subjects with COPD at three UK clinical centres as part of the COPDMAP consortium. RESULTS: The microbiome composition was similar among centres and between stable and exacerbations except for a small significant decrease of Veillonella at exacerbations. The abundance of Moraxella was negatively associated with bacterial alpha diversity. Microbiomes were distinct between exacerbations associated with bacteria versus eosinophilic airway inflammation. Dysbiosis at exacerbations, measured as significant within subject deviation of microbial composition relative to baseline, was present in 41% of exacerbations. Dysbiosis was associated with increased exacerbation severity indicated by a greater fall in forced expiratory volume in one second, forced vital capacity and a greater increase in CAT score, particularly in exacerbations with concurrent eosinophilic inflammation. There was a significant difference of temporal variability of microbial alpha and beta diversity among centres. The variation of beta diversity significantly decreased in those subjects with frequent historical exacerbations. CONCLUSIONS: Microbial dysbiosis is a feature of some exacerbations and its presence, especially in concert with eosinophilic inflammation, is associated with more severe exacerbations indicated by a greater fall in lung function. TRIAL REGISTRATION NUMBER: Results, NCT01620645.
AU  - Wang,Z
AU  - Singh,R
AU  - Miller,BE
AU  - Tal-Singer,R
AU  - Van,Horn S
AU  - Tomsho,L
AU  - Mackay,A
AU  - Allinson,JP
AU  - Webb,AJ
AU  - Brookes,AJ
AU  - George,LM
AU  - Barker,B
AU  - Kolsum,U
AU  - Donnelly,LE
AU  - Belchamber,K
AU  - Barnes,PJ
AU  - Singh,D
AU  - Brightling,CE
AU  - Donaldson,GC
AU  - Wedzicha,JA
AU  - Brown,JR
AU  - COPDMAP
DO  - 10.1136/thoraxjnl-2017-210741
EP  - 338
PY  - 2017///
SN  - 1468-3296
SP  - 331
TI  - Sputum microbiome temporal variability and dysbiosis in chronic obstructive pulmonary disease exacerbations: an analysis of the COPDMAP study
T2  - Thorax
UR  - http://dx.doi.org/10.1136/thoraxjnl-2017-210741
UR  - http://hdl.handle.net/10044/1/55609
VL  - 73
ER  -
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