Imperial College London
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ProfessorPaulKellam

Faculty of Medicine, Department of Infectious Disease

Professor of Virus Genomics
 
 
 
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p.kellam

 
 
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Location

 

460Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thi:2017:10.12688/wellcomeopenres.12429.1,
author = {Thi, Ty Hang V and Thi, Han Ny N and My, Phuc T and Thi, Thanh Tam P and Thao, Huong D and Dang, Trung Nghia H and Tran, Anh Vu N and Thi, Hong Phuong P and Van, Xang N and Dong, N and Nhu, Hiep P and Van, Hung N and Tinh, Hien T and Rabaa, M and Thwaites, GE and Baker, S and Van, Tan L and van, Doorn HR and Berto, A and Boni, MF and Bryant, JE and Phu, BD and Campbell, JI and Carrique-Mas, J and Hung, DM and Huong, DT and Oanh, DT and Day, JN and Tan, DV and Han, DA and Farrar, JJ and Trang, HTT and Long, HB and Duong, HV and Thu, HTK and Cuong, LC and Hung, LM and Phuong, LT and Phuc, LT and Phuong, LT and Luat, LX and Ha, LTT and Chuong, LV and Loan, MTP and Nadjm, B and Bao, NT and Hoa, NT and Tue, NT and Tu, NC and Thuan, ND and Chuyen, NK and An, NN and Vinh, NN and Hung, NQ and Dung, NT and Minh, NT and Binh, NT and Tham, NTH and Tien, NTH and Chuc, NTK and Ngoc, NTL and Ha, NTL and Lien, NTN and Diep, NTN and Nhung, NT and Chau, NTS and Chi, NTY and Trinh, NT and Van, NT and Va},
doi = {10.12688/wellcomeopenres.12429.1},
journal = {Wellcome Open Research},
title = {Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]},
url = {http://dx.doi.org/10.12688/wellcomeopenres.12429.1},
volume = {2},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.
AU  - Thi,Ty Hang V
AU  - Thi,Han Ny N
AU  - My,Phuc T
AU  - Thi,Thanh Tam P
AU  - Thao,Huong D
AU  - Dang,Trung Nghia H
AU  - Tran,Anh Vu N
AU  - Thi,Hong Phuong P
AU  - Van,Xang N
AU  - Dong,N
AU  - Nhu,Hiep P
AU  - Van,Hung N
AU  - Tinh,Hien T
AU  - Rabaa,M
AU  - Thwaites,GE
AU  - Baker,S
AU  - Van,Tan L
AU  - van,Doorn HR
AU  - Berto,A
AU  - Boni,MF
AU  - Bryant,JE
AU  - Phu,BD
AU  - Campbell,JI
AU  - Carrique-Mas,J
AU  - Hung,DM
AU  - Huong,DT
AU  - Oanh,DT
AU  - Day,JN
AU  - Tan,DV
AU  - Han,DA
AU  - Farrar,JJ
AU  - Trang,HTT
AU  - Long,HB
AU  - Duong,HV
AU  - Thu,HTK
AU  - Cuong,LC
AU  - Hung,LM
AU  - Phuong,LT
AU  - Phuc,LT
AU  - Phuong,LT
AU  - Luat,LX
AU  - Ha,LTT
AU  - Chuong,LV
AU  - Loan,MTP
AU  - Nadjm,B
AU  - Bao,NT
AU  - Hoa,NT
AU  - Tue,NT
AU  - Tu,NC
AU  - Thuan,ND
AU  - Chuyen,NK
AU  - An,NN
AU  - Vinh,NN
AU  - Hung,NQ
AU  - Dung,NT
AU  - Minh,NT
AU  - Binh,NT
AU  - Tham,NTH
AU  - Tien,NTH
AU  - Chuc,NTK
AU  - Ngoc,NTL
AU  - Ha,NTL
AU  - Lien,NTN
AU  - Diep,NTN
AU  - Nhung,NT
AU  - Chau,NTS
AU  - Chi,NTY
AU  - Trinh,NT
AU  - Van,NT
AU  - Van,Cuong N
AU  - Van,Kinh NV
AU  - Van,Minh Hoang N
AU  - Van,My N
AU  - Van,Thang N
AU  - Van,Thanh N
AU  - Van,Vinh Chau N
AU  - Ha,My P
AU  - Hong,Anh P
AU  - Thi,Minh Khoa P
AU  - Van,Lao PV
AU  - Van,Minh P
AU  - Van,Be Bay P
AU  - Rahman,M
AU  - Thompson,C
AU  - Thi,Dieu Ngan TTD
AU  - Do,Hoang Nhu T
AU  - Hoang,Minh Chau THM
AU  - Toan,TK
AU  - Thi,Kim Hong T
AU  - Thi,Ngoc Dung T
AU  - Thi,Thanh Thanh T
AU  - Thi,Thuy Minh T
AU  - Thua,Nguyen TT
AU  - Tri,TQ
AU  - Be,Hien V
DO  - 10.12688/wellcomeopenres.12429.1
PY  - 2017///
SN  - 2398-502X
TI  - Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]
T2  - Wellcome Open Research
UR  - http://dx.doi.org/10.12688/wellcomeopenres.12429.1
UR  - http://hdl.handle.net/10044/1/67761
VL  - 2
ER  -
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