Imperial College London
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ProfessorPaulKellam

Faculty of Medicine, Department of Infectious Disease

Professor of Virus Genomics
 
 
 
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p.kellam

 
 
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Location

 

460Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Memish:2014:infdis/jiu292,
author = {Memish, ZA and Al-Tawfiq, JA and Makhdoom, HQ and Assiri, A and Alhakeem, RF and Albarrak, A and Alsubaie, S and Al-Rabeeah, AA and Hajomar, WH and Hussain, R and Kheyami, AM and Almutairi, A and Azhar, EI and Drosten, C and Watson, SJ and Kellam, P and Cotten, M and Zumla, A},
doi = {infdis/jiu292},
journal = {Journal of Infectious Diseases},
pages = {1590--1594},
title = {Respiratory tract samples, viral load, and genome fraction yield in patients with Middle East Respiratory Syndrome},
url = {http://dx.doi.org/10.1093/infdis/jiu292},
volume = {210},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background. Analysis of clinical samples from patients with new viral infections is critical to confirm the diagnosis, to specify the viral load, and to sequence data necessary for characterizing the viral kinetics, transmission, and evolution. We analyzed samples from 112 patients infected with the recently discovered Middle East respiratory syndrome coronavirus (MERS-CoV).Methods. Respiratory tract samples from cases of MERS-CoV infection confirmed by polymerase chain reaction (PCR) were investigated to determine the MERS-CoV load and fraction of the MERS-CoV genome. These values were analyzed to determine associations with clinical sample type.Results. Samples from 112 individuals in which MERS-CoV was detected by PCR were analyzed, of which 13 were sputum samples, 64 were nasopharyngeal swab specimens, 30 were tracheal aspirates, and 3 were bronchoalveolar lavage specimens; 2 samples were of unknown origin. Tracheal aspirates yielded significantly higher MERS-CoV loads, compared with nasopharyngeal swab specimens (P = .005) and sputum specimens (P = .0001). Tracheal aspirates had viral loads similar to those in bronchoalveolar lavage samples (P = .3079). Bronchoalveolar lavage samples and tracheal aspirates had significantly higher genome fraction than nasopharyngeal swab specimens (P = .0095 and P = .0002, respectively) and sputum samples (P = .0009 and P = .0001, respectively). The genome yield from tracheal aspirates and bronchoalveolar lavage samples were similar (P = .1174).Conclusions. Lower respiratory tract samples yield significantly higher MERS-CoV loads and genome fractions than upper respiratory tract samples.
AU  - Memish,ZA
AU  - Al-Tawfiq,JA
AU  - Makhdoom,HQ
AU  - Assiri,A
AU  - Alhakeem,RF
AU  - Albarrak,A
AU  - Alsubaie,S
AU  - Al-Rabeeah,AA
AU  - Hajomar,WH
AU  - Hussain,R
AU  - Kheyami,AM
AU  - Almutairi,A
AU  - Azhar,EI
AU  - Drosten,C
AU  - Watson,SJ
AU  - Kellam,P
AU  - Cotten,M
AU  - Zumla,A
DO  - infdis/jiu292
EP  - 1594
PY  - 2014///
SN  - 0022-1899
SP  - 1590
TI  - Respiratory tract samples, viral load, and genome fraction yield in patients with Middle East Respiratory Syndrome
T2  - Journal of Infectious Diseases
UR  - http://dx.doi.org/10.1093/infdis/jiu292
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000344611200011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/jid/article/210/10/1590/2192931
VL  - 210
ER  -
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