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@article{Burton:2014:europace/euu234,
author = {Burton, RAB and Lee, P and Casero, R and Garny, A and Siedlecka, U and Schneider, JE and Kohl, P and Grau, V},
doi = {europace/euu234},
journal = {Europace},
pages = {86--95},
title = {Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart},
url = {http://dx.doi.org/10.1093/europace/euu234},
volume = {16},
year = {2014}
}

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TY  - JOUR
AB  - AimsCardiac histo-anatomical organization is a major determinant of function. Changes in tissue structure are a relevant factor in normal and disease development, and form targets of therapeutic interventions. The purpose of this study was to test tools aimed to allow quantitative assessment of cell-type distribution from large histology and magnetic resonance imaging- (MRI) based datasets.Methods and resultsRabbit heart fixation during cardioplegic arrest and MRI were followed by serial sectioning of the whole heart and light-microscopic imaging of trichrome-stained tissue. Segmentation techniques developed specifically for this project were applied to segment myocardial tissue in the MRI and histology datasets. In addition, histology slices were segmented into myocytes, connective tissue, and undefined. A bounding surface, containing the whole heart, was established for both MRI and histology. Volumes contained in the bounding surface (called ‘anatomical volume’), as well as that identified as containing any of the above tissue categories (called ‘morphological volume’), were calculated. The anatomical volume was 7.8 cm3 in MRI, and this reduced to 4.9 cm3 after histological processing, representing an ‘anatomical’ shrinkage by 37.2%. The morphological volume decreased by 48% between MRI and histology, highlighting the presence of additional tissue-level shrinkage (e.g. an increase in interstitial cleft space). The ratio of pixels classified as containing myocytes to pixels identified as non-myocytes was roughly 6:1 (61.6 vs. 9.8%; the remaining fraction of 28.6% was ‘undefined’).ConclusionQualitative and quantitative differentiation between myocytes and connective tissue, using state-of-the-art high-resolution serial histology techniques, allows identification of cell-type distribution in whole-heart datasets. Comparison with MRI illustrates a pronounced reduction in anatomical and morphological volumes during histo
AU  - Burton,RAB
AU  - Lee,P
AU  - Casero,R
AU  - Garny,A
AU  - Siedlecka,U
AU  - Schneider,JE
AU  - Kohl,P
AU  - Grau,V
DO  - europace/euu234
EP  - 95
PY  - 2014///
SN  - 1099-5129
SP  - 86
TI  - Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart
T2  - Europace
UR  - http://dx.doi.org/10.1093/europace/euu234
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000209827500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/europace/article/16/suppl_4/iv86/486407
UR  - http://hdl.handle.net/10044/1/84676
VL  - 16
ER  -

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