Imperial College London
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ProfessorSimoneDi Giovanni

Faculty of Medicine, Department of Brain Sciences

James W Harnett Chair in Restorative Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 3178s.di-giovanni

 
 
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Location

 

E505Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Floriddia:2011:10.3791/2803,
author = {Floriddia, E and Nguyen, T and Di, Giovanni S},
doi = {10.3791/2803},
journal = {J Vis Exp},
title = {Chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury.},
url = {http://dx.doi.org/10.3791/2803},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Axons in the central nervous system (CNS) do not regenerate while those in the peripheral nervous system (PNS) do regenerate to a limited extent after injury (Teng et al., 2006). It is recognized that transcriptional programs essential for neurite and axonal outgrowth are reactivated upon injury in the PNS (Makwana et al., 2005). However the tools available to analyze neuronal gene regulation in vivo are limited and often challenging. The dorsal root ganglia (DRG) offer an excellent injury model system because both the CNS and PNS are innervated by a bifurcated axon originating from the same soma. The ganglia represent a discrete collection of cell bodies where all transcriptional events occur, and thus provide a clearly defined region of transcriptional activity that can be easily and reproducibly removed from the animal. Injury of nerve fibers in the PNS (e.g. sciatic nerve), where axonal regeneration does occur, should reveal a set of transcriptional programs that are distinct from those responding to a similar injury in the CNS, where regeneration does not take place (e.g. spinal cord). Sites for transcription factor binding, histone and DNA modification resulting from injury to either PNS or CNS can be characterized using chromatin immunoprecipitation (ChIP). Here, we describe a ChIP protocol using fixed mouse DRG tissue following axonal injury. This powerful combination provides a means for characterizing the pro-regeneration chromatin environment necessary for promoting axonal regeneration.
AU  - Floriddia,E
AU  - Nguyen,T
AU  - Di,Giovanni S
DO  - 10.3791/2803
PY  - 2011///
TI  - Chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury.
T2  - J Vis Exp
UR  - http://dx.doi.org/10.3791/2803
UR  - https://www.ncbi.nlm.nih.gov/pubmed/21808222
ER  -
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