Imperial College London
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Dr Sophie V Morse

Faculty of Engineering, Department of Bioengineering

Imperial College Research Fellow
 
 
 
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Contact

 

sophie.morse11

 
 
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Location

 

B324Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Al:2023:10.1002/psc.3448,
author = {Al, Musaimi O and Morse, SV and Lombardi, L and Serban, S and Basso, A and Williams, DR},
doi = {10.1002/psc.3448},
journal = {Journal of Peptide Science},
pages = {1--9},
title = {Successful synthesis of a glial-specific blood-brain barrier shuttle peptide following a fragment condensation approach on a solid-phase resin},
url = {http://dx.doi.org/10.1002/psc.3448},
volume = {29},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Successful manual synthesis of the TD2.2 peptide acting as a blood-brain barrier shuttle was achieved. TD2.2 was successfully synthesised by sequential condensation of four protected peptide fragments on solid-phase settings, after several unsuccessful attempts using the stepwise approach. These fragments were chosen to minimize the number of demanding amino acids (in terms of coupling, Fmoc removal) in each fragment that are expected to hamper the overall synthetic process. Thus, the hydrophobic amino acids as well as Fmoc-Arg (Pbf)-OH were strategically spread over multiple fragments rather than having them congested in one fragment. This study shows how a peptide that shows big challenges in the synthesis using the common stepwise elongation methodology can be synthesised with an acceptable purity. It also emphasises that choosing the right fragment with certain amino acid constituents is key for a successful synthesis. It is worth highlighting that lower amounts of reagents were required to synthesise the final peptide with an identical purity to that obtained by the automatic synthesiser.
AU  - Al,Musaimi O
AU  - Morse,SV
AU  - Lombardi,L
AU  - Serban,S
AU  - Basso,A
AU  - Williams,DR
DO  - 10.1002/psc.3448
EP  - 9
PY  - 2023///
SN  - 1075-2617
SP  - 1
TI  - Successful synthesis of a glial-specific blood-brain barrier shuttle peptide following a fragment condensation approach on a solid-phase resin
T2  - Journal of Peptide Science
UR  - http://dx.doi.org/10.1002/psc.3448
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35997639
UR  - https://onlinelibrary.wiley.com/doi/10.1002/psc.3448
UR  - http://hdl.handle.net/10044/1/99379
VL  - 29
ER  -
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