Imperial College London
Alternate

ProfessorVictorTybulewicz

Faculty of Medicine, Department of Immunology and Inflammation

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 3796 1612v.tybulewicz Website CV

 
 
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Location

 

L2-2720Francis Crick InstituteThe Francis Crick Institute

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Summary

 

Publications

Citation

BibTex format

@article{Gribble:2013:10.1371/journal.pone.0060482,
author = {Gribble, SM and Wiseman, FK and Clayton, S and Prigmore, E and Langley, E and Yang, F and Maguire, S and Fu, B and Rajan, D and Sheppard, O and Scott, C and Hauser, H and Stephens, PJ and Stebbings, LA and Ng, BL and Fitzgerald, T and Quail, MA and Banerjee, R and Rothkamm, K and Tybulewicz, VLJ and Fisher, EMC and Carter, NP},
doi = {10.1371/journal.pone.0060482},
journal = {PLoS ONE},
title = {Massively parallel sequencing reveals the complex structure of an irradiated human chromosome on a mouse background in the Tc1 model of down syndrome},
url = {http://dx.doi.org/10.1371/journal.pone.0060482},
volume = {8},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Down syndrome (DS) is caused by trisomy of chromosome 21 (Hsa21) and presents a complex phenotype that arises from abnormal dosage of genes on this chromosome. However, the individual dosage-sensitive genes underlying each phenotype remain largely unknown. To help dissect genotype – phenotype correlations in this complex syndrome, the first fully transchromosomic mouse model, the Tc1 mouse, which carries a copy of human chromosome 21 was produced in 2005. The Tc1 strain is trisomic for the majority of genes that cause phenotypes associated with DS, and this freely available mouse strain has become used widely to study DS, the effects of gene dosage abnormalities, and the effect on the basic biology of cells when a mouse carries a freely segregating human chromosome. Tc1 mice were created by a process that included irradiation microcell-mediated chromosome transfer of Hsa21 into recipient mouse embryonic stem cells. Here, the combination of next generation sequencing, array-CGH and fluorescence in situ hybridization technologies has enabled us to identify unsuspected rearrangements of Hsa21 in this mouse model; revealing one deletion, six duplications and more than 25 de novo structural rearrangements. Our study is not only essential for informing functional studies of the Tc1 mouse but also (1) presents for the first time a detailed sequence analysis of the effects of gamma radiation on an entire human chromosome, which gives some mechanistic insight into the effects of radiation damage on DNA, and (2) overcomes specific technical difficulties of assaying a human chromosome on a mouse background where highly conserved sequences may confound the analysis. Sequence data generated in this study is deposited in the ENA database, Study Accession number: ERP000439.
AU  - Gribble,SM
AU  - Wiseman,FK
AU  - Clayton,S
AU  - Prigmore,E
AU  - Langley,E
AU  - Yang,F
AU  - Maguire,S
AU  - Fu,B
AU  - Rajan,D
AU  - Sheppard,O
AU  - Scott,C
AU  - Hauser,H
AU  - Stephens,PJ
AU  - Stebbings,LA
AU  - Ng,BL
AU  - Fitzgerald,T
AU  - Quail,MA
AU  - Banerjee,R
AU  - Rothkamm,K
AU  - Tybulewicz,VLJ
AU  - Fisher,EMC
AU  - Carter,NP
DO  - 10.1371/journal.pone.0060482
PY  - 2013///
SN  - 1932-6203
TI  - Massively parallel sequencing reveals the complex structure of an irradiated human chromosome on a mouse background in the Tc1 model of down syndrome
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0060482
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000317563300006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/57998
VL  - 8
ER  -
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