Imperial College London
Alternate

DrXavierDidelot

Faculty of Medicine, School of Public Health

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3622x.didelot

 
 
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Location

 

G30Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cao:2015:10.1136/gutjnl-2014-307345,
author = {Cao, Q and Didelot, X and Wu, Z and Li, Z and He, L and Li, Y and Ni, M and You, Y and Lin, X and Li, Z and Gong, Y and Zheng, M and Zhang, M and Liu, J and Wang, W and Bo, X and Falush, D and Wang, S and Zhang, J},
doi = {10.1136/gutjnl-2014-307345},
journal = {Gut},
pages = {554--561},
title = {Progressive genomic convergence of two Helicobacter pylori strains during mixed infection of a patient with chronic gastritis},
url = {http://dx.doi.org/10.1136/gutjnl-2014-307345},
volume = {64},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Objective To study the detailed nature of genomic microevolution during mixed infection with multiple Helicobacter pylori strains in an individual. Design We sampled 18 isolates from a single biopsy from a patient with chronic gastritis and nephritis. Whole-genome sequencing was applied to these isolates, and statistical genetic tools were used to investigate their evolutionary history. Results The genomes fall into two clades, reflecting colonisation of the stomach by two distinct strains, and these lineages have accumulated diversity during an estimated 2.8 and 4.2 years of evolution. We detected about 150 clear recombination events between the two clades. Recombination between the lineages is a continuous ongoing process and was detected on both clades, but the effect of recombination in one clade was nearly an order of magnitude higher than in the other. Imputed ancestral sequences also showed evidence of recombination between the two strains prior to their diversification, and we estimate that they have both been infecting the same host for at least 12 years. Recombination tracts between the lineages were, on average, 895 bp in length, and showed evidence for the interspersion of recipient sequences that has been observed in in vitro experiments. The complex evolutionary history of a phage-related protein provided evidence for frequent reinfection of both clades by a single phage lineage during the past 4 years. Conclusions Whole genome sequencing can be used to make detailed conclusions about the mechanisms of genetic change of H. pylori based on sampling bacteria from a single gastric biopsy.
AU  - Cao,Q
AU  - Didelot,X
AU  - Wu,Z
AU  - Li,Z
AU  - He,L
AU  - Li,Y
AU  - Ni,M
AU  - You,Y
AU  - Lin,X
AU  - Li,Z
AU  - Gong,Y
AU  - Zheng,M
AU  - Zhang,M
AU  - Liu,J
AU  - Wang,W
AU  - Bo,X
AU  - Falush,D
AU  - Wang,S
AU  - Zhang,J
DO  - 10.1136/gutjnl-2014-307345
EP  - 561
PY  - 2015///
SN  - 0017-5749
SP  - 554
TI  - Progressive genomic convergence of two Helicobacter pylori strains during mixed infection of a patient with chronic gastritis
T2  - Gut
UR  - http://dx.doi.org/10.1136/gutjnl-2014-307345
VL  - 64
ER  -
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