BibTex format

author = {Wu, N and Silva, LM and Liu, Y and Zhang, Y and Gao, C and Zhang, F and Fu, L and Peng, Y and Linhardt, R and Kawasaki, T and Mulloy, B and Chai, W and Feizi, T},
doi = {10.1074/mcp.RA119.001309},
journal = {Molecular and Cellular Proteomics},
pages = {1981--2002},
title = {Glycan markers of human stem cells assigned with beam search arrays},
url = {},
volume = {18},
year = {2019}

RIS format (EndNote, RefMan)

AB - Glycan antigens recognized by monoclonal antibodies have served as stem cell markers. To understand regulation of their biosynthesis and their roles in stem cell behavior precise assignments are required. We have applied state-of-the-art glycan array technologies to compare the glycans bound by five antibodies that recognize carbohydrates on human stem cells. These are: FC10.2, TRA-1-60, TRA-1-81, anti-i and R-10G. Microarray analyses with a panel of sequence-defined glycans corroborate that FC10.2, TRA-1-60, TRA-1-81 recognize the type 1-(Galβ-3GlcNAc)-terminating backbone sequence, Galβ-3GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and anti-i, the type 2-(Galβ-4GlcNAc) analog, Galβ-4GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and we determine substituents they can accommodate. They differ from R-10G, which requires sulfate. By Beam Search approach, starting with an antigen-positive keratan sulfate polysaccharide, followed by targeted iterative microarray analyses of glycan populations released with keratanases and mass spectrometric monitoring, R-10G is assigned as a mono-sulfated type 2 chain with 6-sulfation at the penultimate N-acetylglucosamine, Galβ-4GlcNAc(6S)β-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc. Microarray analyses using newly synthesized glycans corroborate the assignment of this unique determinant raising questions regarding involvement as a ligand in the stem cell niche.
AU - Wu,N
AU - Silva,LM
AU - Liu,Y
AU - Zhang,Y
AU - Gao,C
AU - Zhang,F
AU - Fu,L
AU - Peng,Y
AU - Linhardt,R
AU - Kawasaki,T
AU - Mulloy,B
AU - Chai,W
AU - Feizi,T
DO - 10.1074/mcp.RA119.001309
EP - 2002
PY - 2019///
SN - 1535-9476
SP - 1981
TI - Glycan markers of human stem cells assigned with beam search arrays
T2 - Molecular and Cellular Proteomics
UR -
UR -
UR -
VL - 18
ER -